| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Wang, Lei
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (23/23 displayed)
- 2023An analysis of rotationally moulded sandwich structure’s repeated impact properties
- 2023Fusion of Ni Plating on CP-Titanium by Electron Beam Single-Track Scanning: Toward a New Approach for Fabricating TiNi Self-Healing Shape Memory Coating
- 2022Green synthesis of Ag/Fe3O4 nanoparticles using Mentha longifolia flower extract: evaluation of its antioxidant and anti-lung cancer effects
- 2022An atomistic simulation study of rapid solidification kinetics and crystal defects in dilute Al–Cu alloyscitations
- 2022Multiscale analysis of crystalline defect formation in rapid solidification of pure aluminium and aluminium–copper alloyscitations
- 2022Reprocessed materials used in rotationally moulded sandwich structures for enhancing environmental sustainability: low-velocity impact and flexure-after-impact responsescitations
- 2022Multiscale analysis of crystal defect formation in rapid solidification of pure aluminium and aluminium-copper alloys
- 2022Mechanically Flexible Thermoelectric Hybrid Thin Films by Introduction of PEDOT:PSS in Nanoporous Ca3Co4O9citations
- 2021On the use of limestone calcined clay cement (LC<sup>3</sup>) in high-strength strain-hardening cement-based composites (HS-SHCC)citations
- 2021Bimetallic effects on Zn-Cu electrocatalysts enhance activity and selectivity for the conversion of CO2 to COcitations
- 2021Growth and optical properties of CaxCoO2 thin filmscitations
- 2021Guiding the Catalytic Properties of Copper for Electrochemical CO2 Reduction by Metal Atom Decoration.citations
- 2020The role of zinc in metakaolin-based geopolymerscitations
- 2020Unconventional valley-dependent optical selection rules and landau level mixing in bilayer graphenecitations
- 2019Magic continuum in twisted bilayer WSe2
- 2019An investigation of low velocity impact properties of rotationally molded skin–foam–skin sandwich structurecitations
- 2018Fracture toughness of rotationally molded polyethylene and polypropylenecitations
- 2016Nonlinear Generation of Vector Beams from AlGaAs Nanoantennascitations
- 2016Electron optics with p-n junctions in ballistic graphenecitations
- 2014Charge Control And Wettability Alteration At Solid-liquid Interfacescitations
- 2011A Common Genetic Variant in the 3′-UTR of Vacuolar H <sup>+</sup> -ATPase <i>ATP6V0A1</i> Creates a Micro-RNA Motif to Alter Chromogranin A Processing and Hypertension Riskcitations
- 2008Heritability and Genome-Wide Linkage in US and Australian Twins Identify Novel Genomic Regions Controlling Chromogranin Acitations
- 2007Polysulfide networks. in Situ formation and characterization of the elastomeric behaviorcitations
Places of action
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article
A Common Genetic Variant in the 3′-UTR of Vacuolar H <sup>+</sup> -ATPase <i>ATP6V0A1</i> Creates a Micro-RNA Motif to Alter Chromogranin A Processing and Hypertension Risk
Abstract
<jats:sec><jats:title>Background—</jats:title><jats:p>The catecholamine release-inhibitor catestatin and its precursor chromogranin A (CHGA) may constitute “intermediate phenotypes” in the analysis of genetic risk for cardiovascular disease such as hypertension. Previously, the vacuolar H<jats:sup>+</jats:sup>-ATPase subunit gene<jats:italic>ATP6V0A1</jats:italic>was found within the confidence interval for linkage with catestatin secretion in a genome-wide study, and its 3′-UTR polymorphism T+3246C (rs938671) was associated with both catestatin processing from CHGA and population blood pressure. We explored the molecular mechanism of this effect by experiments with transfected chimeric photoproteins in chromaffin cells.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results—</jats:title><jats:p>Placing the<jats:italic>ATP6V0A1</jats:italic>3′-UTR downstream of a luciferase reporter, we found that the C (variant) allele decreased overall gene expression. The 3′-UTR effect was verified by coupled in vitro transcription/translation of the entire/intact human<jats:italic>ATP6V0A1</jats:italic>mRNA. Chromaffin granule pH, monitored by fluorescence of CHGA/EGFP chimera during vesicular H<jats:sup>+</jats:sup>-ATPase inhibition by bafilomycin A1, was more easily perturbed during coexpression of the<jats:italic>ATP6V0A1</jats:italic>3′-UTR C-allele than the T-allele. After bafilomycin A1 treatment, the ratio of CHGA precursor to its catestatin fragments in PC12 cells was substantially diminished, though the qualitative composition of such fragments was not affected (on immunoblot or matrix-assisted laser desorption ionization (MALDI) mass spectrometry). Bafilomycin A1 treatment also decreased exocytotic secretion from the regulated pathway, monitored by a CHGA chimera tagged with embryonic alkaline phosphatase. 3′-UTR T+3246C created a binding motif for micro-RNA hsa-miR-637; cotransfection of hsa-miR-637 precursor or antagomir/inhibitor oligonucleotides yielded the predicted changes in expression of luciferase reporter/<jats:italic>ATP6V0A1</jats:italic>-3′-UTR plasmids varying at T+3246C.</jats:p></jats:sec><jats:sec><jats:title>Conclusions—</jats:title><jats:p>The results suggest a series of events whereby<jats:italic>ATP6V0A1</jats:italic>3′-UTR variant T+3246C functioned:<jats:italic>ATP6V0A1</jats:italic>expression probably was affected through differential micro-RNA effects, altering vacuolar pH and consequently CHGA processing and exocytotic secretion.</jats:p></jats:sec>