• Özcan, Nermin
• Atar, Necip

Abstract

<jats:p>Interleukin-6 (IL-6) as a pro-inflammatory cytokine demonstrate a critical role in the inflammatory response. Especially, the high levels of IL-6 measured in plasma have been associated with pathological inflammation. In this report, new molecularly imprinting biosensor on graphene quantum dots (GQDs)/functionalized multi-walled carbon nanotubes (f-MWCNTs) composite were prepared for IL-6 protein detection. The structures of GQDs, f-MWCNTs and GQDs/f-MWCNTs composite were highlighted by scanning electron microscope (SEM), transmission electron microscopy (TEM), raman spectroscopy, UV–vis spectroscopy, fourier transform infrared spectroscopy (FTIR), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and X-ray diffraction (XRD) method. Surface morphology characterization shows the nanoporous cavities as an effective biosensing area. IL-6 protein imprinted electrode was prepared on GQDs/f-MWCNTs composite in the presence of 100.0 mM pyrrole containing 25.0 mM IL-6 protein. 0.01–2.0 pg ml<jats:sup>−1</jats:sup> and 0.0030 pg ml<jats:sup>−1</jats:sup> were found as linearity range and the detection limit (LOD) for analytical application in plasma samples. Finally, the validated biosensor was examined in terms of stability, repeatability and reproducibility.</jats:p>

Topics

• surface
• Carbon
• scanning electron microscopy
• x-ray diffraction
• nanotube
• composite
• transmission electron microscopy
• electrochemical-induced impedance spectroscopy
• Raman spectroscopy
• Fourier transform infrared spectroscopy
• quantum dot
• cyclic voltammetry