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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Górski, Łukasz
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Topics
Publications (7/7 displayed)
- 2017Phosphorothioated oligonucleotides as receptor layers in electrochemical detection of uranyl cationcitations
- 2016Electrochemical Determination of Hg2+ Using Electrodes Modified with Peptide Nucleic Acidcitations
- 2015Electrochemical oligonucleotide-based biosensor for the determination of lead ioncitations
- 2013Development of silicon-based electrochemical transducerscitations
- 2013Oligonucleotide-Based Electrochemical Biosensor for Hg2+ Using Methylene Blue as a Redox Indicatorcitations
- 2009Influence of inner transducer properties on EMF response and stability of solid-contact anion-selective membrane electrodes based on metalloporphyrin ionophorescitations
- 2004Polymeric membrane electrodes with enhanced fluoride selectivity using Zr(IV)-porphyrins functioning as neutral carrierscitations
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article
Phosphorothioated oligonucleotides as receptor layers in electrochemical detection of uranyl cation
Abstract
The feasibility of using gold electrodes modified with phosphorothioated oligonucleotides (PTOs) for determination of uranyl cation is described. PTOs are able of anchoring to gold surface via phosphorothioate linkers, present in its backbone. This approach is advantageous due to lack of the necessity of chemical activation before use and low cost. It was shown that the sensor sensitivity highly dependents on the incubation time in the sample solution. The developed biosensor demonstrated high selectivity toward UO22+ ion with minor interferences from other metal ions. A linear relationship between the changes in current signal derived from methylene blue – used as a redox marker – and the uranyl ion concentration up to 100 nmol•L−1 was obtained, with a detection limit of 14.7 nmol•L−1. In order to characterize the morphology of gold surface modified with DNA-SH or PTO, atomic force microscopy (AFM) was used. Interactions between UO22+ and the receptor layer (PTO) were studied using square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS).