| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Górski, Łukasz
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (7/7 displayed)
- 2017Phosphorothioated oligonucleotides as receptor layers in electrochemical detection of uranyl cationcitations
- 2016Electrochemical Determination of Hg2+ Using Electrodes Modified with Peptide Nucleic Acidcitations
- 2015Electrochemical oligonucleotide-based biosensor for the determination of lead ioncitations
- 2013Development of silicon-based electrochemical transducerscitations
- 2013Oligonucleotide-Based Electrochemical Biosensor for Hg2+ Using Methylene Blue as a Redox Indicatorcitations
- 2009Influence of inner transducer properties on EMF response and stability of solid-contact anion-selective membrane electrodes based on metalloporphyrin ionophorescitations
- 2004Polymeric membrane electrodes with enhanced fluoride selectivity using Zr(IV)-porphyrins functioning as neutral carrierscitations
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article
Oligonucleotide-Based Electrochemical Biosensor for Hg2+ Using Methylene Blue as a Redox Indicator
Abstract
Gold electrodesmodified with short-chain ss-DNA oligonucleotides were used for trace-level voltammetric determination ofmercury cation. External redox marker, methylene blue, was employed for these measurements. Due to the competition between Hg2+ and methylene blue, current signal decreases proportionally to the analyte concentration. It was shown that 5-SH-(CH2)6-(T)15−3 oligonucleotide, when employed as a receptor layer, induces the strongest electrochemical signal, among all tested DNA sequences. Electrochemical impedance spectroscopy measurements confirmed a specific interaction between the oligonucleotide receptor layer and Hg2+ ions. Proposed biosensor exhibited significant selectivity toward mercury ion, with strong discrimination of other common metal cations. The remarkably low detection limit of 4.63 nmol · L−1 for Hg2+ ions was calculated for proposed biosensor. The usefulness of developed biosensor was verified by its application for analysis of Hg2+ in aqueous samples.