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Jarczewska, Marta Maria
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article
Oligonucleotide-Based Electrochemical Biosensor for Hg2+ Using Methylene Blue as a Redox Indicator
Abstract
Gold electrodesmodified with short-chain ss-DNA oligonucleotides were used for trace-level voltammetric determination ofmercury cation. External redox marker, methylene blue, was employed for these measurements. Due to the competition between Hg2+ and methylene blue, current signal decreases proportionally to the analyte concentration. It was shown that 5-SH-(CH2)6-(T)15−3 oligonucleotide, when employed as a receptor layer, induces the strongest electrochemical signal, among all tested DNA sequences. Electrochemical impedance spectroscopy measurements confirmed a specific interaction between the oligonucleotide receptor layer and Hg2+ ions. Proposed biosensor exhibited significant selectivity toward mercury ion, with strong discrimination of other common metal cations. The remarkably low detection limit of 4.63 nmol · L−1 for Hg2+ ions was calculated for proposed biosensor. The usefulness of developed biosensor was verified by its application for analysis of Hg2+ in aqueous samples.