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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Liu, Jie
Eindhoven University of Technology
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (14/14 displayed)
- 2024Data Management in Multicountry Consortium Studies: The Enterics For Global Health (EFGH) <i>Shigella</i> Surveillance Study Examplecitations
- 2023Enantiopure Dinaphtho[2,3-b:2,3-f]thieno[3,2-b]thiophenes: Reaching High Magnetoresistance Effect in OFETscitations
- 2023Enantiopure Dinaphtho[2,3-b:2,3-f]thieno[3,2-b]thiophenes: Reaching High Magnetoresistance Effect in OFETs.citations
- 2023Enantiopure Dinaphtho[2,3‐<i>b</i>:2,3‐<i>f</i>]thieno[3,2‐<i>b</i>]thiophenes: Reaching High Magnetoresistance Effect in OFETscitations
- 2021Abstract 449: A standard operating procedure for the curation of gene fusions
- 2021Unraveling the Complexity of Supramolecular Copolymerization Dictated by Triazine-Benzene Interactionscitations
- 2020Sputtering of LiF and other halide crystals in the electronic energy loss regimecitations
- 2020Exploring structural changes, manufacturing, joining, and repair of intermetallic γ-TiAl-based alloys: Recent progress enabled by in situ synchrotron X-Ray techniquescitations
- 2018Radiation-induced extreme elastic and inelastic interactions in concentrated solid solutionscitations
- 2016Phase Transformation and Residual Stress in a Laser Beam Spot-Welded TiAl-Based Alloycitations
- 2015Multiphysics Characterization of an Albian Post-Salt Carbonate Reservoir, Brazil
- 2015Integrated Microfluidic Card with TaqMan Probes and High-Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genescitations
- 2014Effect of Post-weld Heat Treatment on Microstructure and Mechanical Properties of Laser Beam Welded TiAl-based Alloycitations
- 2013A Laboratory-Developed TaqMan Array Card for Simultaneous Detection of 19 Enteropathogenscitations
Places of action
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article
Integrated Microfluidic Card with TaqMan Probes and High-Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genes
Abstract
<jats:title>ABSTRACT</jats:title><jats:p>Genotypic methods for drug susceptibility testing of<jats:named-content content-type="genus-species">Mycobacterium tuberculosis</jats:named-content>are desirable to speed the diagnosis and proper therapy of tuberculosis (TB). However, the numbers of genes and polymorphisms implicated in resistance have proliferated, challenging diagnostic design. We developed a microfluidic TaqMan array card (TAC) that utilizes both sequence-specific probes and high-resolution melt analysis (HRM), providing two layers of detection of mutations. Twenty-seven primer pairs and 40 probes were designed to interrogate 3,200 base pairs of critical regions of the<jats:italic>inhA</jats:italic>,<jats:italic>katG</jats:italic>,<jats:italic>rpoB</jats:italic>,<jats:italic>embB</jats:italic>,<jats:italic>rpsL</jats:italic>,<jats:italic>rrs</jats:italic>,<jats:italic>eis</jats:italic>,<jats:italic>gyrA</jats:italic>,<jats:italic>gyrB</jats:italic>, and<jats:italic>pncA</jats:italic>genes. The method was evaluated on 230 clinical M. tuberculosis isolates from around the world, and it yielded 96.1% accuracy (2,431/2,530) in comparison to that of Sanger sequencing and 87% accuracy in comparison to that of the slow culture-based susceptibility testing. This TAC-HRM method integrates assays for 10 genes to yield fast, comprehensive, and accurate drug susceptibility results for the 9 major antibiotics used to treat TB and could be deployed to improve treatment outcomes.</jats:p><jats:p><jats:bold>IMPORTANCE</jats:bold>Multidrug-resistant tuberculosis threatens global tuberculosis control efforts. Optimal therapy utilizes susceptibility test results to guide individualized treatment regimens; however, the susceptibility testing methods in use are technically difficult and slow. We developed an integrated TaqMan array card method with high-resolution melt analysis that interrogates 10 genes to yield a fast, comprehensive, and accurate drug susceptibility result for the 9 major antituberculosis antibiotics.</jats:p>