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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Liu, Jie
Eindhoven University of Technology
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (14/14 displayed)
- 2024Data Management in Multicountry Consortium Studies: The Enterics For Global Health (EFGH) <i>Shigella</i> Surveillance Study Examplecitations
- 2023Enantiopure Dinaphtho[2,3-b:2,3-f]thieno[3,2-b]thiophenes: Reaching High Magnetoresistance Effect in OFETscitations
- 2023Enantiopure Dinaphtho[2,3-b:2,3-f]thieno[3,2-b]thiophenes: Reaching High Magnetoresistance Effect in OFETs.citations
- 2023Enantiopure Dinaphtho[2,3‐<i>b</i>:2,3‐<i>f</i>]thieno[3,2‐<i>b</i>]thiophenes: Reaching High Magnetoresistance Effect in OFETscitations
- 2021Abstract 449: A standard operating procedure for the curation of gene fusions
- 2021Unraveling the Complexity of Supramolecular Copolymerization Dictated by Triazine-Benzene Interactionscitations
- 2020Sputtering of LiF and other halide crystals in the electronic energy loss regimecitations
- 2020Exploring structural changes, manufacturing, joining, and repair of intermetallic γ-TiAl-based alloys: Recent progress enabled by in situ synchrotron X-Ray techniquescitations
- 2018Radiation-induced extreme elastic and inelastic interactions in concentrated solid solutionscitations
- 2016Phase Transformation and Residual Stress in a Laser Beam Spot-Welded TiAl-Based Alloycitations
- 2015Multiphysics Characterization of an Albian Post-Salt Carbonate Reservoir, Brazil
- 2015Integrated Microfluidic Card with TaqMan Probes and High-Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genescitations
- 2014Effect of Post-weld Heat Treatment on Microstructure and Mechanical Properties of Laser Beam Welded TiAl-based Alloycitations
- 2013A Laboratory-Developed TaqMan Array Card for Simultaneous Detection of 19 Enteropathogenscitations
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article
A Laboratory-Developed TaqMan Array Card for Simultaneous Detection of 19 Enteropathogens
Abstract
<jats:title>ABSTRACT</jats:title><jats:p>The TaqMan Array Card (TAC) system is a 384-well singleplex real-time PCR format that has been used to detect multiple infection targets. Here we developed an enteric TaqMan Array Card to detect 19 enteropathogens, including viruses (adenovirus, astrovirus, norovirus GII, rotavirus, and sapovirus), bacteria (<jats:named-content content-type="genus-species">Campylobacter jejuni/C. coli</jats:named-content>,<jats:named-content content-type="genus-species">Clostridium difficile</jats:named-content>,<jats:named-content content-type="genus-species">Salmonella</jats:named-content>,<jats:named-content content-type="genus-species">Vibrio cholerae</jats:named-content>, diarrheagenic<jats:named-content content-type="genus-species">Escherichia coli</jats:named-content>strains including enteroaggregative<jats:named-content content-type="genus-species">E. coli</jats:named-content>[EAEC], enterotoxigenic<jats:named-content content-type="genus-species">E. coli</jats:named-content>[ETEC], enteropathogenic<jats:named-content content-type="genus-species">E. coli</jats:named-content>[EPEC], and Shiga-toxigenic<jats:named-content content-type="genus-species">E. coli</jats:named-content>[STEC]),<jats:named-content content-type="genus-species">Shigella</jats:named-content>/enteroinvasive<jats:named-content content-type="genus-species">E. coli</jats:named-content>(EIEC), protozoa (<jats:named-content content-type="genus-species">Cryptosporidium</jats:named-content>,<jats:named-content content-type="genus-species">Giardia lamblia</jats:named-content>, and<jats:named-content content-type="genus-species">Entamoeba histolytica</jats:named-content>), and helminths (<jats:named-content content-type="genus-species">Ascaris lumbricoides</jats:named-content>and<jats:named-content content-type="genus-species">Trichuris trichiura</jats:named-content>), as well as two extrinsic controls to monitor extraction and amplification efficiency (the bacteriophage MS2 and phocine herpesvirus). Primers and probes were newly designed or adapted from published sources and spotted onto microfluidic cards. Fecal samples were spiked with extrinsic controls, and DNA and RNA were extracted using the QiaAmp Stool DNA minikit and the QuickGene RNA Tissue kit, respectively, and then mixed with Ag-Path-ID One Step real-time reverse transcription-PCR (RT-PCR) reagents and loaded into cards. PCR efficiencies were between 90% and 105%, with linearities of 0.988 to 1. The limit of detection of the assays in the TAC was within a 10-fold difference from the cognate assays performed on plates. Precision testing demonstrated a coefficient of variation of below 5% within a run and 14% between runs. Accuracy was evaluated for 109 selected clinical specimens and revealed an average sensitivity and specificity of 85% and 77%, respectively, compared with conventional methods (including microscopy, culture, and immunoassay) and 98% and 96%, respectively, compared with our laboratory-developed PCR-Luminex assays. This TAC allows fast, accurate, and quantitative detection of a broad spectrum of enteropathogens and is well suited for surveillance or clinical purposes.</jats:p>