| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Boissinot, Maurice
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (4/4 displayed)
- 2012Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from watercitations
- 2009Internal Control for Nucleic Acid Testing Based on the Use of Purified <i>Bacillus atrophaeus</i> subsp. <i>globigii</i> Sporescitations
- 2007Specific Magnetic Bead–Based Capture of Genomic DNA from Clinical Samples: Application to the Detection of Group B Streptococci in Vaginal/Anal Swabscitations
- 2002Rapid Detection of Shiga Toxin-Producing Bacteria in Feces by Multiplex PCR with Molecular Beacons on the Smart Cyclercitations
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article
Internal Control for Nucleic Acid Testing Based on the Use of Purified <i>Bacillus atrophaeus</i> subsp. <i>globigii</i> Spores
Abstract
<jats:p>Commonly used internal controls (ICs) to monitor the efficiency of nucleic acid testing (NAT) assays do not allow verification of nucleic acid extraction efficiency. Since microbial cells are often difficult to lyse, it is important to ensure that nucleic acids are efficiently extracted from any target organism. For this purpose, we developed a cellular IC based on the use of nonpathogenic<jats:italic>Bacillus</jats:italic>spores. Purified<jats:italic>Bacillus atrophaeus</jats:italic>subsp.<jats:italic>globigii</jats:italic>(referred to hereafter as simply<jats:italic>B. atrophaeus</jats:italic>) spores were added to vaginal and anal samples, which were then subjected to rapid DNA extraction and subsequent PCR amplification. The proof of concept of this cellular IC was made through the use of both manual and automated DNA extraction methods, using vaginal or anal samples spiked with<jats:italic>B. atrophaeus</jats:italic>spores, combined with a multiplex real-time PCR assay for the specific detection of group B streptococci (GBS) and<jats:italic>B. atrophaeus</jats:italic>. The performance of the cellular IC was compared to that of a standard IC plasmid added to PCRs. Approximately 500<jats:italic>B. atrophaeus</jats:italic>spores per PCR was found to be optimal since this did not interfere significantly with GBS detection for either DNA extraction method and yielded reproducible amplification and/or detection of<jats:italic>B. atrophaeus</jats:italic>genomic DNA serving as an IC template. Performance of the cellular IC was comparable to that of the standard IC. This novel IC system using nonpathogenic and hard-to-lyse<jats:italic>B. atrophaeus</jats:italic>spores allowed validation of both the DNA extraction procedure and the amplification and detection process. Use of a spore-based control also provides a universal control for microbial cell lysis.</jats:p>