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Abele-Horn, Marianne
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article
Microbiological Evaluation of the New VITEK 2 <i>Neisseria</i> - <i>Haemophilus</i> Identification Card
Abstract
<jats:title>ABSTRACT</jats:title><jats:p>VITEK 2 is an automated identification system for diverse bacterial and fungal species. A new card (the<jats:italic>Neisseria</jats:italic>-<jats:italic>Haemophilus</jats:italic>[NH] card) for the identification of<jats:italic>Neisseria</jats:italic>spp.,<jats:italic>Haemophilus</jats:italic>spp., and other fastidious gram-negative or gram-variable microorganisms has been developed, but its performance in a routine clinical laboratory has not yet been evaluated. In this study, a total of 188 bacterial strains belonging to the genera<jats:italic>Actinobacillus</jats:italic>,<jats:italic>Campylobacter</jats:italic>,<jats:italic>Capnocytophaga</jats:italic>,<jats:italic>Cardiobacterium</jats:italic>,<jats:italic>Eikenella</jats:italic>,<jats:italic>Gardnerella</jats:italic>,<jats:italic>Haemophilus</jats:italic>,<jats:italic>Kingella</jats:italic>,<jats:italic>Moraxella</jats:italic>, and<jats:italic>Neisseria</jats:italic>were investigated. The NH card was able to identify 171 strains (91%) correctly without the need for extra tests; one strain (0.5%) was misidentified, and five strains (2.7%) could not be classified. Eleven strains (5.8%) were identified with a low level of discrimination, and simple additional tests were required to increase the correct-identification rate to 96.8%. The results were available within 6 h. Based on these results, the new VITEK 2 NH card appears to be a good method for the identification of diverse groups of fastidious organisms, which would otherwise require testing with multiple systems. However, more work is needed to evaluate the performance of VITEK 2 with regard to<jats:italic>Haemophilus</jats:italic>,<jats:italic>Actinobacillus</jats:italic>,<jats:italic>Cardiobacterium</jats:italic>,<jats:italic>Eikenella</jats:italic>, and<jats:italic>Kingella</jats:italic>bacteria because of the insufficient number of strains tested in this study. Moreover, further reduction of the detection time would be desirable.</jats:p>