| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Wang, Hui
Queen's University Belfast
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (23/23 displayed)
- 2024CNC-Machined and 3D-Printed Metal G-band Diplexers for Earth Observation Applicationscitations
- 2023Evaluation of 3D printed monolithic G-band waveguide componentscitations
- 2023Machine learning on spectral data from miniature devices for food quality analysis - a case study
- 2023Halide-guided active site exposure in bismuth electrocatalysts for selective CO2 conversion into formic acidcitations
- 2023Halide-guided active site exposure in bismuth electrocatalysts for selective CO2 conversion into formic acid
- 2022A 3D printed 300 GHz waveguide cavity filter by micro laser sinteringcitations
- 2022D-band waveguide diplexer fabricated using micro laser sinteringcitations
- 2022Waste‐Derived Copper‐Lead Electrocatalysts for CO<sub>2</sub> Reductioncitations
- 2022Waste-Derived Copper-Lead Electrocatalysts for CO 2 Reductioncitations
- 2022Waste-Derived Copper-Lead Electrocatalysts for CO2 Reduction
- 2021125 GHz frequency doubler using a waveguide cavity produced by stereolithographycitations
- 2021Trileucine as a dispersibility enhancer of spray-dried inhalable microparticlescitations
- 2016Thermoelectric Properties of Polymeric Mixed Conductorscitations
- 2014Self-assembled nano- to micron-size fibers from molten R11Ni4In9 intermetallicscitations
- 2014Semi-metallic polymerscitations
- 2014Semi-metallic polymerscitations
- 2013Spatially resolved investigation of strain and composition variations in (In,Ga)N/GaN epilayerscitations
- 2013Exploring suitable oligoamines for phantom ring-closing condensation polymerization with guanidine hydrochloridecitations
- 2013A novel method to measure diffusion coefficients in porous metal-organic frameworks (vol 12, pg 8093, 2010)
- 2010A metadata-based approach for multimedia service mashup in IMS
- 2010A novel method to measure diffusion coefficients in porous metal-organic frameworkscitations
- 2010Phenotypic characterization of shewanella oneidensis MR-1 under aerobic and anaerobic growth conditions by using fourier transform infrared spectroscopy and high-performance liquid chromatography analysescitations
- 2010Impact of silver(I) on the metabolism of Shewanella oneidensiscitations
Places of action
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article
Phenotypic characterization of shewanella oneidensis MR-1 under aerobic and anaerobic growth conditions by using fourier transform infrared spectroscopy and high-performance liquid chromatography analyses
Abstract
Shewanella oneidensis is able to conserve energy for growth by reducing a wide variety of terminal electron acceptors during anaerobic respiration, including several environmentally hazardous pollutants. This bacterium employs various electron transfer mechanisms for anaerobic respiration, including cell-bound reductases and secreted redox mediators. The aim of this study was to develop rapid tools for profiling the key metabolic changes associated with these different growth regimes and physiological responses. Initial experiments focused on comparing cells grown under aerobic and anaerobic conditions. Fourier transform infrared (FT-IR) spectroscopy with cluster analysis showed that there were significant changes in the metabolic fingerprints of the cells grown under these two culture conditions. FT-IR spectroscopy clearly differentiated cells of S. oneidensis MR-1 cultured at various growth points and cells grown using different electron acceptors, resulting in different phenotypic trajectories in the cluster analysis. This growth-related trajectory analysis is applied successfully for the first time, here with FT-IR spectroscopy, to investigate the phenotypic changes in contrasting S. oneidensis cells. High-performance liquid chromatography (HPLC) was also used to quantify the concentrations of flavin compounds, which have been identified recently as extracellular redox mediators released by a range of Shewanella species. The partial least-squares regression (PLSR) multivariate statistical technique was combined with FT-IR spectroscopy to predict the concentrations of the flavins secreted by cells of S. oneidensis MR-1, suggesting that this combination could be used as a rapid alternative to conventional chromatographic methods for analysis of flavins in cell cultures. Furthermore, coupling of the FT-IR spectroscopy and HPLC techniques appears to offer a potentially useful tool for rapid characterization of the Shewanella cell metabolome in various process environments Copyright © 2010, American Society ror Microbiology. All Rights Reserved.