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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Dybko, Artur
Warsaw University of Technology
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (9/9 displayed)
- 20192D Ti2C (MXene) as a novel highly efficient and selective agent for photothermal therapycitations
- 2017Microfluidic system for monitoring of cardiac (H9C2) cell proliferation
- 2016Evaluation of nanoencapsulated verteporfin's cytotoxicity using a microfluidic systemcitations
- 2012Effect of a high surface-to-volume ratio on fluorescence-based assayscitations
- 2007AgI-Ag2O-V2O5 glasses as ion-to-electron transducers for the construction of all-solid-state microelectrodescitations
- 2006Fabrication of ceramic-polymer microstructure for capillary electrophoresis with usage of photosensitive paste and thick film technology
- 2006Ammonium- and nitrate-selective all-solid-state microelectrodes based on AgI-Ag2O-V2O5 glass transducer
- 2004Towards advanced chemical microsensors—an overviewcitations
- 2001Multi-ion analysis based on versatile sensor headcitations
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document
Microfluidic system for monitoring of cardiac (H9C2) cell proliferation
Abstract
The paper presents the application of electrical impedance spectroscopy (EIS) analysis for investigation of cardiac cell (H9C2-rat cardiomyoblast) proliferation after verapamil hydrochloride exposure. For this purpose, two different PDMS/glass microsystems with circular microchamber and longitudinal microchannel integrated with Pt/Al electrodes were used. The microchambers were fabricated in PDMS using photolithography and replica moulding techniques. Pt/Al electrodes were fabricated on a 4-inch glass substrate using Physical Vapor Deposition (PVD). Solution of verapamil hydrochloride was continuously introduced into the microsystems with H9C2 cell culture (a flow rate of 1 μl/min) for 72 h. The impedance spectra were recorded from 100 Hz to 1 MHz. We confirmed that impedance spectroscopy can be used for non-invasive, label-free and real-time analysis of cardiac cells proliferation based on cells dielectric properties and biological structure.