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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Muir, Ben
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (10/10 displayed)
- 2020Lipid Nanodiscs via Ordered Copolymerscitations
- 2020Analyzing 3D Hyperspectral ToF-SIMS Depth Profile Data Using Self-Organizing Map-Relational Perspective Mappingcitations
- 2018Distinguishing chemically similar polyamide materials with ToF-SIMS using self-organizing maps and a universal data matrixcitations
- 2017Limitations with solvent exchange methods for synthesis of colloidalfullerenescitations
- 2016Probing the interfacial structure of bilayer plasma polymer films via neutron reflectometry
- 2016Investigation of the growth mechanisms of diglyme plasma polymers on amyloid fibril networkscitations
- 2016Chromium functionalized diglyme plasma polymer coating enhances enzyme-linked immunosorbent assay performancecitations
- 2015Protic ionic liquids (PILs) nanostructure and physicochemical properties: development of high-throughput methodology for PIL creation and property screenscitations
- 2014Water-dispersible magnetic carbon nanotubes as T2-weighted MRI contrast agentscitations
- 2014A study of the initial film growth of PEG-like plasma polymer films via XPS and NEXAFScitations
Places of action
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article
Chromium functionalized diglyme plasma polymer coating enhances enzyme-linked immunosorbent assay performance
Abstract
Ensuring the optimum orientation, conformation, and density of substrate-bound antibodies is critical for the success of sandwich enzyme-linked immunosorbent assays (ELISAs). In this work, the authors utilize a diethylene glycol dimethyl ether plasma polymer (DGpp) coating, functionalized with chromium within a 96 well plate for the enhanced immobilization of a capture antibody. For an equivalent amount of bound antibody, a tenfold improvement in the ELISA signal intensity is obtained on the DGpp after incubation with chromium, indicative of improved orientation on this surface. Time-of-flight secondary-ion-mass-spectrometry (ToF-SIMS) and principal component analysis were used to probe the molecular species at the surface and showed ion fragments related to lysine, methionine, histidine, and arginine coupled to chromium indicating candidate antibody binding sites. A combined x-ray photoelectron spectroscopy and ToF-SIMS analysis provided a surface molecular characterization that demonstrates antibody binding via the chromium complex. The DGpp+Cr surface treatment holds great promise for improving the efficacy of ELISAs.