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Widmer, Franco
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article
Residual polymerase activity‐induced bias in terminal restriction fragment length polymorphism analysis
Abstract
<jats:title>Summary</jats:title><jats:p>Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5′‐overhang restriction site fill‐in and addition of a terminal A may bias genetic profiling and genotyping of microbial communities. Efficient removal of polymerases retained original fragment sizes and significantly reduced this profiling bias in soil bacterial communities.</jats:p>