| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Camilleri, Josette
University of Birmingham
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (23/23 displayed)
- 2024Proposing new standards for testing solubility of pulp preservation materialscitations
- 2024Chemical, antibacterial and cytotoxic properties of four different endodontic sealer leachates over timecitations
- 2024Leaching and cytotoxicity of bismuth oxide in ProRoot MTAcitations
- 2024Characterization and Assessment of Physical Properties of 3 Single Syringe Hydraulic Cement-based Sealerscitations
- 2023Editorial
- 2022Surface characteristics and bacterial adhesion of endodontic cements.citations
- 2022Surface characteristics and bacterial adhesion of endodontic cements ; ENEngelskEnglishSurface characteristics and bacterial adhesion of endodontic cementscitations
- 2021Effect of different manipulations on the physical, chemical and microstructural characteristics of Biodentinecitations
- 2021PRILE 2021 guidelines for reporting laboratory studies in endodontologycitations
- 2020Antimicrobial and ultrastructural properties of root canal filling materials exposed to bacterial challengecitations
- 2020Characterization of heat resistant hydraulic sealer for warm vertical obturationcitations
- 2020Classification of hydraulic cements used in dentistrycitations
- 2019Bioactivity potential of Portland cement in regenerative endodontic procedurescitations
- 2019Investigation of the effect of the water to powder ratio on hydraulic cement propertiescitations
- 2018Surface Microstructural Changes and Release of Ions from Dental Metal Alloy Removable Prostheses in Patients Suffering from Acid Refluxcitations
- 2018The effect of mixing method on tricalcium silicate-based cementcitations
- 2018Assessment of corrosion resistance of cast cobalt- and nickel-chromium dental alloys in acidic environmentscitations
- 2017Antimicrobial activity of ProRoot MTA in contact with bloodcitations
- 2017Bonding over Dentin Replacement Materialscitations
- 2017Will Bioceramics be the Future Root Canal Filling Materials?citations
- 2016Assessment of the interaction of Portland cement-based materials with blood and tissue fluids using an animal modelcitations
- 2015Effect of sterilization techniques prior to antimicrobial testing on physical properties of dental restorative materialscitations
- 2014Color stability of white mineral trioxide aggregate in contact with hypochlorite solutioncitations
Places of action
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article
Leaching and cytotoxicity of bismuth oxide in ProRoot MTA
Abstract
Aim: The present study examined the leaching and cytotoxicity of bismuth from ProRoot MTA and aimed to identify whether bismuth leaching was affected by the cement base and the immersion regime used. <br/><br/>Methodology: The leaching profile of bismuth was examined from ProRoot MTA and compared with hydroxyapatite containing 20% bismuth oxide as well as hydroxyapatite and tricalcium silicate to investigate whether bismuth release changed depending on the cement base. Bismuth leaching was determined after 30 and 180 days of ageing immersed in Dulbecco's modified Eagle's medium (DMEM) using mass spectroscopy (ICP‐MS). The media were either unchanged or regularly replenished. The pH, surface microstructure and phase changes of aged materials were assessed. Wistar rat femoral bone marrow stromal cells (BMSCs) and cutaneous fibroblasts were isolated, cultured and seeded for cell counting (trypan blue live/dead) after exposure to non‐aged, 30‐ and 180‐days‐aged samples in regularly replenished DMEM. Aged DMEM in contact with materials was also used to culture BMSCs to investigate the effect of material leachates on the cells. Gene expression analysis was also carried out after direct exposure of cells to non‐aged materials. Differences between groups were statistically tested at a significance level of 5%. <br/><br/>Results: All materials exhibited alterations after immersion in DMEM and this increased with longer exposure times. The bismuth leached from ProRoot MTA as detected by ICP‐MS. Aged ProRoot MTA samples exhibited a black discolouration and surface calcium carbonate deposition. ProRoot MTA influenced cell counts after direct exposure and its 180‐days leachates reduced BMSC viability. After direct BMSC contact with non‐aged ProRoot MTA an upregulation of metallothionein (MT1 and MT2A) expression and down‐regulation of collagen‐1a (Col‐1a) and bone sialoprotein (BSP) expression was identified. <br/><br/>Conclusions: Bismuth leaching was observed throughout 180‐days observation period from all materials containing bismuth oxide. This negatively influenced cell viability and gene expression associated with bismuth exposure. This is the first study to report that metallothionein gene expression was influenced by exposure to ProRoot MTA.