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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Petrov, R. H. | Madrid |
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Casati, R. |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Alahi, M. E. E.
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Development of molecular imprinted polymer interdigital sensor for C-terminal telopeptide of type I collagen
Abstract
<p>This paper presents a label-free and non-invasive technique for selective detection of C-terminal telopeptide type I collagen (CTx-I) by employing Electrochemical Impedance Spectroscopy to measure sample impedance. Molecular imprinted polymer, containing artificial recognition sites for CTx-was prepared by precipitation polymerization using CTx-I peptide as a template, methacrylic acid as a functional monomer and ethylene glycol methacrylate as the cross-linker. A high penetration depth planar interdigital sensor was functionalized by a self-assembled monolayer along with the synthesized MIP. Different concentrations of CTx-I sample solutions were tested using the proposed sensing system. High-Performance Liquid chromatography diode array system was used to validate the results.</p>