| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Spiliopoulou, Maria
University of Patras
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (7/7 displayed)
- 2021High-throughput macromolecular polymorph screening via an NMR and X-ray powder diffraction synergistic approach: the case of human insulin co-crystallized with resorcinol derivativescitations
- 2020Insulin polymorphism induced by two polyphenols: new crystal forms and advances in macromolecular powder diffractioncitations
- 2019Unit-cell response of tetragonal hen egg white lysozyme upon controlled relative humidity variationcitations
- 2019Revisiting the structure of a synthetic somatostatin analogue for peptide drug designcitations
- 2018<i>In situ</i>detection of a novel lysozyme monoclinic crystal form upon controlled relative humidity variationcitations
- 2017Dengue virus 3 NS5 methyltransferase domain: expression, purification, crystallization and first structural data from microcrystalline specimenscitations
- 2016Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insightscitations
Places of action
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article
Insulin polymorphism induced by two polyphenols: new crystal forms and advances in macromolecular powder diffraction
Abstract
<jats:p>This study focuses on the polymorphism of human insulin (HI) upon the binding of the phenolic derivatives<jats:italic>p</jats:italic>-coumaric acid or<jats:italic>trans</jats:italic>-resveratrol over a wide pH range. The determination of the structural behaviour of HI via X-ray powder diffraction (XRPD) and single-crystal X-ray diffraction (SCXRD) is reported. Four distinct polymorphs were identified, two of which have not been reported previously. The intermediate phase transitions are discussed. One of the novel monoclinic polymorphs displays the highest molecular packing among insulin polymorphs of the same space group to date; its structure was elucidated by SCXRD. XRPD data collection was performed using a variety of instrumental setups and a systematic comparison of the acquired data is presented. A laboratory diffractometer was used for screening prior to high-resolution XRPD data collection on the ID22 beamline at the European Synchrotron Radiation Facility. Additional measurements for the most representative samples were performed on the X04SA beamline at the Swiss Light Source (SLS) using the MYTHEN II detector, which allowed the detection of minor previously untraceable impurities and dramatically improved the<jats:italic>d</jats:italic>-spacing resolution even for poorly diffracting samples.</jats:p>