• Penn, Andrew C.
• Sainlos, Matthieu
• Ducros, Mathieu
• Marais, Sébastien
• Lemoigne, Cécile
• Choquet, Daniel
• Nowacka, Agata
• Daburon, Sophie
• Breillat, Christelle
• Bessa-Neto, Diogo
• Zieger, Hanna L.
• Getz, Angela

Abstract

<jats:title>Abstract</jats:title><jats:p>Synaptic responses adapt to fast repetitive inputs during bursts of neuronal network activity over timescales of milliseconds to seconds, either transiently facilitating or depressing. This high-frequency stimulus-dependent short-term synaptic plasticity (HF-STP) relies on a number of molecular processes that collectively endow synapses with filtering properties for information processing, optimized for the transmission of certain input frequencies and patterns in distinct circuits<jats:sup>1–3</jats:sup>. Changes in HF-STP are traditionally thought to stem from changes in pre-synaptic transmitter release<jats:sup>1,2</jats:sup>, but post-synaptic modifications in receptor biophysical properties or surface diffusion also regulate HF-STP<jats:sup>4–11</jats:sup>. A major challenge in understanding synapse function is to decipher how pre- and post-synaptic mechanisms synergistically tune synaptic transmission efficacy during HF-STP, and to determine how neuronal activity modifies post-synaptic signal computation and integration to diversify neuronal circuit function. Here, taking advantage of new molecular tools to directly visualize glutamate release<jats:sup>12</jats:sup>and specifically manipulate the surface diffusion of endogenous AMPAR in intact circuits<jats:sup>13</jats:sup>, we define the respective contributions of pre-synaptic glutamate release, AMPAR desensitization and surface mobility to frequency-dependent synaptic adaptation. We demonstrate that post-synaptic gain control and signal integration capacity in synaptic networks is influenced by synapse-specific differences in AMPAR desensitization and diffusion-trapping characteristics that are shaped by molecular signaling events recruited during LTP.</jats:p>

Topics

• impedance spectroscopy
• surface
• mobility
• plasticity