| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Tozzi, Gianluca
University of Greenwich
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2024Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cellscitations
- 2023Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cellscitations
- 2022Nonlinear micro finite element models based on digital volume correlation measurements predict early microdamage in newly formed bonecitations
- 2021Multi-scale mechanical and morphological characterisation of sintered porous magnesium-based scaffolds for bone regeneration in critical-sized defectscitations
- 2021Time-resolved in situ synchrotron-microCTcitations
- 2020Flexural resistance of CAD/CAM blocks. Part 3: polymer-based restorative materials for permanent restorations
- 2020Low-cycle full-field residual strains in cortical bone and their influence on tissue fracture evaluated via in situ stepwise and continuous X-ray computed tomographycitations
- 2019Anisotropic crack propagation and deformation in dentin observed by four-dimensional X-ray nano-computed tomographycitations
- 2019Sustained release from injectable composite gels loaded with silver nanowires designed to combat bacterial resistance in bone regeneration applicationscitations
- 2017Precision of digital volume correlation approaches for strain analysis in bone imaged with micro-computed tomography at different dimensional levelscitations
- 20174D printing biomimetic tissue structures using correlative approaches
- 2016Morphological and mechanical biomimetic bone structurescitations
- 2012Compressive fatigue behaviour of bovine cancellous bone and bone analogous materials under multi-step loading conditionscitations
Places of action
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document
Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells
Abstract
<jats:title>Abstract</jats:title><jats:p>Autograft or metal implants are routinely used in skeletal repair but can fail to provide a long-term clinical resolution, emphasising the need for a functional biomimetic tissue engineering alternative. An attractive sustainable opportunity for tissue regeneration would be the application of human bone waste tissue for the synthesis of a material ink for 3D bioprinting of skeletal tissue.</jats:p><jats:p>The use of human bone extracellular matrix (bone-ECM) offers an exciting potential for the development of an appropriate micro-environment for human bone marrow stromal cells (HBMSCs) to proliferate and differentiate along the osteogenic lineage. Extrusion-based deposition was mediated by the blending of human bone-ECM (B) with nanoclay (L, Laponite<jats:sup>®</jats:sup>) and alginate (A) polymer, to engineer a novel material ink (LAB). The inclusion of nanofiller and polymeric material increased the rheological, printability, and drug retention properties and, critically, the preservation of HBMSCs viability upon printing. The composite human bone-ECM-based 3D constructs containing vascular endothelial growth factor (VEGF) enhanced vascularisation following implantation in an<jats:italic>ex vivo</jats:italic>chick chorioallantoic membrane (CAM) model. Addition of bone morphogenetic protein-2 (BMP-2) with HBMSCs further enhanced vascularisation together with mineralisation after only 7 days.</jats:p><jats:p>The current study demonstrates the synergistic combination of nanoclay with biomimetic materials, (alginate and bone-ECM) to support the formation of osteogenic tissue both<jats:italic>in vitro</jats:italic>and<jats:italic>ex vivo</jats:italic>and offers a promising novel 3D bioprinting approach to personalised skeletal tissue repair.</jats:p><jats:sec><jats:title>Graphical Abstract</jats:title><jats:p>Engineering nanoclay-based bone ECM novel bioink for bone regeneration. Human bone trabecular tissue was demineralised, decellularised and blended with nanoclay (Laponite®) and alginate after digestion. The resulting ink was investigated for printability following rheological and filament fusion investigation. The microstructural arrangement of the blends was examined together with viability and functionality of bioprinted HBMSCs. Finally, the ability of the novel blend to support drug release ex vivo in a CAM model was determined confirming the potential of the bone ECM ink to support bone formation.</jats:p><jats:fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="536074v1_ufig1" position="float" orientation="portrait" /></jats:fig></jats:sec>