• Cortese, Yvonne J.

Abstract

<jats:p><jats:bold>Background:</jats:bold> There is currently no standard established <jats:italic>in vitro</jats:italic> model to test the efficacy of intermittent catheters to prevent or control introduction/movement of bacteria into the urethra during device insertion. This study aimed to address this issue by developing a reproducible agar based <jats:italic>in vitro</jats:italic> urethral model.</jats:p><jats:p><jats:bold>Method:</jats:bold> A novel <jats:italic>in vitro</jats:italic> model and testing method was developed to quantify the displacement of bacterial growth after intermittent catheter insertion.The urethral model consists primarily of a preformed channel within a specifically formulated agar based matrix. The urethra model was inoculated at one side of the channel to act as the urethral meatus, a catheter was then inserted. After incubation the bacteria within the urethra channel was quantified.</jats:p><jats:p><jats:bold>Results:</jats:bold> Once optimised, the model produced reliable and reproducible results with both <jats:italic>E. coli</jats:italic> and <jats:italic>S. aureus</jats:italic> (P≥0.265). The model was used to test three different intermittent catheter types. When compared to the growth control there was a significant difference in bacterial distribution when inserting an uncoated (P≤0.001) or hydrophilic coated (P≤0.009) catheter; there was no significant difference when a prototype catheter was inserted with either bacterial species used (P≥0.423).</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> These findings support the hypothesis that a single catheter insertion can initiate a catheter-associated urinary tract infection. The <jats:italic>in vitro</jats:italic> urethra model and associated methodology provide a new research tool for the development and validation of emerging technologies in urological healthcare.</jats:p>

Topics

• impedance spectroscopy