| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Kellomäki, Minna
Tampere University of Technology
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (31/31 displayed)
- 2024A comprehensive study on rheological properties of photocrosslinkable gallol-metal complexed hyaluronic acid-based biomaterial inkscitations
- 2023Comprehensive characterisation of the compressive behaviour of hydrogels using a new modelling procedure and redefining compression testingcitations
- 2023Interpretable machine learning methods for monitoring polymer degradation in extrusion of polylactic acidcitations
- 2023Hydrolytic degradation of polylactide/polybutylene succinate blends with bioactive glasscitations
- 2023Chemical interactions in composites of gellan gum and bioactive glass: self-crosslinking and in vitro dissolutioncitations
- 2022Fractal-like Hierarchical CuO Nano/Microstructures for Large-Surface-to-Volume-Ratio Dip Catalystscitations
- 2022Injectable and self-healing biobased composite hydrogels as future anticancer therapeutic biomaterialscitations
- 2021Comprehensive characterisation of the compressive behaviour of hydrogels using a new modelling procedure and redefining compression testingcitations
- 2021Impact of glass composition on hydrolytic degradation of polylactide/bioactive glass compositescitations
- 2021In vitro degradation testing of hydrogels – concept and case study of gellan gum degradation in water
- 2020Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based toolscitations
- 2020Materials and Orthopedic Applications for Bioresorbable Inductively Coupled Resonance Sensorscitations
- 2020A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samplescitations
- 2019Hydrolysis and drug release from poly(ethylene glycol)-modified lactone polymers with open porositycitations
- 2019Gas-foamed poly(lactide-co-glycolide) and poly(lactide-co-glycolide) with bioactive glass fibres demonstrate insufficient bone repair in lapine osteochondral defectscitations
- 2019Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studiescitations
- 2019Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studiescitations
- 2018Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesiscitations
- 2018Cell response to round and star-shaped polylactide fibers
- 2018Bioresorbable Conductive Wire with Minimal Metal Contentcitations
- 2017In vitro degradation of borosilicate bioactive glass and poly(L-lactide-co-ε-caprolactone) composite scaffoldscitations
- 2016Piezoelectric sensitivity of a layered film of chitosan and cellulose nanocrystalscitations
- 2016Inductively coupled passive resonance sensor for monitoring biodegradable polymers in vitrocitations
- 2016X-ray microtomography of collagen and polylactide samples in liquidscitations
- 2014Direct laser writing of synthetic poly(amino acid) hydrogels and poly(ethylene glycol) diacrylates by two-photon polymerizationcitations
- 2013Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiationcitations
- 2013An in vitro study of composites of poly(L-lactide-co-e-caprolactone), ß-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitiscitations
- 2012Processing and sustained in vitro release of rifampicin containing composites to enhance the treatment of osteomyelitiscitations
- 2011Knitted polylactide 96/4 L/D structures and scaffolds for tissue engineeringcitations
- 2011Investigation of the optimal processing parameters for picosecond laser-induced microfabrication of a polymer-ceramic hybrid materialcitations
- 2008Nano-, Pico- and femtosecond laser machining of bioabsorbable polymers and biomedical composites
Places of action
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article
Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools
Abstract
<p>Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO2 processed poly(l-lactide-co-ɛ-caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.</p>