| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Hyttinen, Jari Aarne Kalevi
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (11/11 displayed)
- 2024Vat photopolymerization of biomimetic bone scaffolds based on Mg, Sr, Zn-substituted hydroxyapatitecitations
- 2023Improvements in Maturity and Stability of 3D iPSC-Derived Hepatocyte-like Cell Culturescitations
- 2023Hydrolytic degradation of polylactide/polybutylene succinate blends with bioactive glasscitations
- 2021Retrieval of the conductivity spectrum of tissues in vitro with novel multimodal tomographycitations
- 2020Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based toolscitations
- 2020A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samplescitations
- 2017Crystallization and sintering of borosilicate bioactive glasses for application in tissue engineeringcitations
- 2017In vitro degradation of borosilicate bioactive glass and poly(L-lactide-co-ε-caprolactone) composite scaffoldscitations
- 2016Texture descriptors ensembles enable image-based classification of maturation of human stem cell-derived retinal pigmented epitheliumcitations
- 2016X-ray microtomography of collagen and polylactide samples in liquidscitations
- 2015μCT based assessment of mechanical deformation of designed PTMC scaffoldscitations
Places of action
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article
Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools
Abstract
<p>Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO2 processed poly(l-lactide-co-ɛ-caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.</p>