| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Miettinen, Susanna
Tampere University
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (19/19 displayed)
- 2024Cornea-Specific Human Adipose Stem Cell-Derived Extracellular Matrix for Corneal Stroma Tissue Engineeringcitations
- 2024Vat photopolymerization of biomimetic bone scaffolds based on Mg, Sr, Zn-substituted hydroxyapatite : Effect of sintering temperaturecitations
- 2024Boron substitution in silicate bioactive glass scaffolds to enhance bone differentiation and regenerationcitations
- 2024Vat photopolymerization of biomimetic bone scaffolds based on Mg, Sr, Zn-substituted hydroxyapatitecitations
- 2023Improvements in Maturity and Stability of 3D iPSC-Derived Hepatocyte-like Cell Culturescitations
- 2021Retrieval of the conductivity spectrum of tissues in vitro with novel multimodal tomographycitations
- 2020Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based toolscitations
- 2020Materials and Orthopedic Applications for Bioresorbable Inductively Coupled Resonance Sensorscitations
- 2020A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samplescitations
- 2018Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesiscitations
- 2018Cell response to round and star-shaped polylactide fibers
- 2016Differentiation of adipose stem cells seeded towards annulus fibrosus cells on a designed poly(trimethylene carbonate) scaffold prepared by stereolithographycitations
- 2016Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds Is a Potential Approach for Tendon Tissue Engineeringcitations
- 2016UV Cross-Linkable Graphene/Poly(trimethylene Carbonate) Composites for 3D Printing of Electrically Conductive Scaffoldscitations
- 2013Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiationcitations
- 2010Oil Induced Swelling in Thermoelastic Materials
- 2010Porous polylactide/beta-tricalcium phosphate composite scaffolds for tissue engineering applicationscitations
- 2009Calcium phosphate surface treatment of bioactive glass causes a delay in early osteogenic differentiation of adipose stem cellscitations
- 2009Characterization of zinc-releasing three-dimensional bioactive glass scaffolds and their effect on human adipose stem cell proliferation and osteogenic differentiationcitations
Places of action
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article
Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools
Abstract
<p>Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO2 processed poly(l-lactide-co-ɛ-caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.</p>