| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Fu, Z.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (15/15 displayed)
- 2023Using Selective Electron Beam Melting to Enhance the High-Temperature Strength and Creep Resistance of NiAl–28Cr–6Mo In Situ Compositescitations
- 2022Uncovering translation roadblocks during the development of a synthetic tRNA.citations
- 2020Preparation of Fe-Co-B-Si-Nb bulk metallic glasses by laser powder bed fusion: Microstructure and propertiescitations
- 2020Electron beam based additive manufacturing of Fe<inf>3</inf>Al based iron aluminides – Processing window, microstructure and propertiescitations
- 2020Effect of the oxygen content of pure copper powder on selective electron beam meltingcitations
- 2019Advanced process strategy to realize microducts free of powder using selective electron beam meltingcitations
- 2019Selective electron beam melting of an aluminum bronze: Microstructure and mechanical propertiescitations
- 20193D Printed Copper Waveguides by Selective Electron Beam Melting Process for E-Bandcitations
- 2019Immediate development of processing windows for selective electron beam melting using layerwise monitoring via backscattered electron detectioncitations
- 2018Crack healing of ferrosilicochromium-filled polymer-derived ceramic compositescitations
- 2018Paper-Derived Ferroelectric Ceramics: A Feasibility Studycitations
- 2017Robocasting of carbon-alumina core-shell composites using co-extrusioncitations
- 2017Laminated Object Manufacturing of in-situ synthesized MAX-phase compositescitations
- 2017Micro- and macroscopic design of alumina ceramics by robocastingcitations
- 2013Three-dimensional printing of SiSiC lattice truss structurescitations
Places of action
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article
Uncovering translation roadblocks during the development of a synthetic tRNA.
Abstract
Ribosomes are remarkable in their malleability to accept diverse aminoacyl-tRNA substrates from both the same organism and other organisms or domains of life. This is a critical feature of the ribosome that allows the use of orthogonal translation systems for genetic code expansion. Optimization of these orthogonal translation systems generally involves focusing on the compatibility of the tRNA, aminoacyl-tRNA synthetase, and a non-canonical amino acid with each other. As we expand the diversity of tRNAs used to include non-canonical structures, the question arises as to the tRNA suitability on the ribosome. Specifically, we investigated the ribosomal translation of allo-tRNAUTu1, a uniquely shaped (9/3) tRNA exploited for site-specific selenocysteine insertion, using single-molecule fluorescence. With this technique we identified ribosomal disassembly occurring from translocation of allo-tRNAUTu1 from the A to the P site. Using cryo-EM to capture the tRNA on the ribosome, we pinpointed a distinct tertiary interaction preventing fluid translocation. Through a single nucleotide mutation, we disrupted this tertiary interaction and relieved the translation roadblock. With the continued diversification of genetic code expansion, our work highlights a targeted approach to optimize translation by distinct tRNAs as they move through the ribosome.