• Michaelsen, Terje E.
• Chen, Xinyong
• Paulsen, Berit S.
• Morris, Gordon
• Yamada, Haruki
• Inngjerdingen, Marit
• Diallo, Drissa
• Harding, Stephen E.
• Allen, Stephanie
• Matsumoto, Tsukasa
• Kenne, Lennart
• Patel, Trushar R.
• Inngjerdingen, Kari T.

Abstract

<p>The aim of this paper was to further elucidate the structure and the immunomodulating properties of the pectic polymer GOA2, previously isolated from Glinus oppositifolius. Enzymatic treatment of GOA2 by endo -α-d-(1→4)-polygalacturonase led to the isolation of three pectic subunits, GOA2-I, GOA2-II, and GOA2-III, in addition to oligogalacturonides. GOA2-I was shown to consist of 1,2-linked Rha p and 1,4-linked Gal p A in an approximately 1:1 ratio, and NMR-analysis showed that the monomers were linked together in a strictly alternating manner. The galactose units in GOA2-I were found as terminal-, 1,3-, 1,6-, 1,4-, 1,3,4-, and 1,3,6-linked residues, while the arabinofuranosyl existed mainly as terminal- and 1,5-linked units. A rhamnogalacturonan-I type structure was suggested being the predominant part of GOA2-I. According to linkage analysis GOA2-II and GOA2-III contained glycosidic linkages characteristic for rhamnogalacturonan-II type structures. GOA2 was shown by sedimentation velocity in the analytical ultracentrifuge, to have a broad degree of polydispersity with a mode s_20,w value of ∼1.9 S, results reinforced by atomic force microscopy measurements. The polydispersity, as manifested by the proportion of material with s_20,w &gt; 3 S, decreased significantly with enzyme treatment. The abilities of GOA2, GOA2-I, GOA2-II, and GOA2-III to induce the proliferation of B cells, and to exhibit complement fixing activities were tested. In both test systems, GOA2-I showed significantly greater effects compared to its native pectin GOA2. GOA2-I was in addition shown to exhibit a more potent intestinal immune stimulating activity compared to GOA2. The ability of GOA2 to induce secretion of proinflammatory cytokines was examined. Marked upregulations in mRNA for IL-1βB2; from rat macrophages and IFN-γ from NK cells were found.</p>

Topics

• polymer
• atomic force microscopy
• Nuclear Magnetic Resonance spectroscopy
• polydispersity