• Ashraf, Rizwan
• Mushtaq, Muhammad
• Akram, Sumia
• Huma, Rahila
• Hasany, Syeda Mariam

Abstract

<jats:title>Abstract</jats:title><jats:p>This study presents a pragmatic and easily scalable maceration-mediated liquid–liquid extraction (MMLLE) and reverse-phase high-performance liquid chromatography (RP-HPLC)-based determination of Silybins from plant material (Curcuma longa L.). The processing of calibration standards revealed that the RP-HPLC method was linear over a concentration range of 1–100 μg/mL with regression coefficient (R2) &amp;gt; 0.9950, limit of detection 0.02 μg/mL and limit of quantification &amp;lt;0.07 μg/mL. The optimum chromatographic conditions resolved Silybin A, Silybin B, Isosilybin A and Isosilybin B within 5 min of analysis time. The reproducible recovery rates of spiked flavonolignans (96.24–115.40%) from quality controls established the effectiveness of MMLLE procedure prior to HPLC determination. The real-time analysis revealed the presence of silybins in C. longa roots. The results further endorse that MMLLE prior to chromatographic determination may provide a more pragmatic analytical solution for the analysis/isolation of silybins.</jats:p>

Topics

• phase
• High-performance liquid chromatography
• liquid–liquid extraction