| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Dawson, Jonathan
University of Southampton
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2023Branched copolymer surfactants impart thermoreversible gelation to LAPONITE® gels †
- 2023Branched copolymer surfactants impart thermoreversible gelation to LAPONITE® gels
- 2022Determination of protoplast growth properties using quantitative single-cell tracking analysiscitations
- 2021Nanocomposite clay-based bioinks for skeletal tissue engineeringcitations
- 2020Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formationcitations
- 2020Bisphosphonate nanoclay edge-site interactions facilitate hydrogel self-assembly and sustained growth factor localizationcitations
- 2020Nanoclay-polyamine composite hydrogel for topical delivery of nitric oxide gas via innate gelation characteristics of laponitecitations
- 2020Nanoclay-polyamine composite hydrogel for topical delivery of nitric oxide gas via innate gelation characteristics of laponitecitations
- 2020Nanoclay-based 3D printed scaffolds promote vascular ingrowth ex vivo and generate bone mineral tissue in vitro and in vivo
- 2019Printing bone in a gel: using nanocomposite bioink to print functionalised bone scaffoldscitations
- 2019Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinkscitations
- 2018Clay nanoparticles for regenerative medicine and biomaterial designcitations
- 2013A tissue engineering strategy for the treatment of avascular necrosis of the femoral headcitations
Places of action
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article
Nanoclay-based 3D printed scaffolds promote vascular ingrowth ex vivo and generate bone mineral tissue in vitro and in vivo
Abstract
<p>Acellular soft hydrogels are not ideal for hard tissue engineering given their poor mechanical stability, however, in combination with cellular components offer significant promise for tissue regeneration. Indeed, nanocomposite bioinks provide an attractive platform to deliver human bone marrow stromal cells (HBMSCs) in three dimensions producing cell-laden constructs that aim to facilitate bone repair and functionality. Here we present the in vitro, ex vivo and in vivo investigation of bioprinted HBMSCs encapsulated in a nanoclay-based bioink to produce viable and functional three-dimensional constructs. HBMSC-laden constructs remained viable over 21 d in vitro and immediately functional when conditioned with osteogenic media. 3D scaffolds seeded with human umbilical vein endothelial cells (HUVECs) and loaded with vascular endothelial growth factor (VEGF) implanted ex vivo into a chick chorioallantoic membrane (CAM) model showed integration and vascularisation after 7 d of incubation. In a pre-clinical in vivo application of a nanoclay-based bioink to regenerate skeletal tissue, we demonstrated bone morphogenetic protein-2 (BMP-2) absorbed scaffolds produced extensive mineralisation after 4 weeks (p < 0.0001) compared to the drug-free and alginate controls. In addition, HBMSC-laden 3D printed scaffolds were found to significantly (p < 0.0001) support bone tissue formation in vivo compared to acellular and cast scaffolds. These studies illustrate the potential of nanoclay-based bioink, to produce viable and functional constructs for clinically relevant skeletal tissue regeneration.</p>