• Hussain, Rabia
• Boccaccini, Ar
• Avcu, Egemen
• Irfan, Muhammad
• Rehman, Muhammad Atiq Ur
• Awais, Muhammad
• Khan, Ahmad
• Akhtar, Memoona
• Nazneen, Arooba

Abstract

<jats:title>Abstract</jats:title><jats:p>The present work focuses on developing 5% w/v oxidized alginate (alginate di aldehyde, ADA)-7.5% w/v gelatin (GEL) hydrogels incorporating 0.25% w/v silk fibroin (SF) and loaded with 0.3% w/v Cu-Ag doped mesoporous bioactive glass nanoparticles (Cu-Ag MBGNs). The microstructural, mechanical, and biological properties of the composite hydrogels were characterized in detail. The porous microstructure of the developed ADA-GEL based hydrogels was confirmed by scanning electron microscopy, while the presence of Cu-Ag MBGNs in the synthesized hydrogels was determined using energy dispersive x-ray spectroscopy. The incorporation of 0.3% w/v Cu-Ag MBGNs reduced the mechanical properties of the synthesized hydrogels, as investigated using micro-tensile testing. The synthesized ADA-GEL loaded with 0.25% w/v SF and 0.3% w/v Cu-Ag MBGNs showed a potent antibacterial effect against <jats:italic>Escherichia coli</jats:italic> and <jats:italic>Staphylococcus aureus</jats:italic>. Cellular studies using the NIH3T3-E1 fibroblast cell line confirmed that ADA-GEL films incorporated with 0.3% w/v Cu-Ag MBGNs exhibited promising cellular viability as compared to pure ADA-GEL (determined by WST-8 assay). The addition of SF improved the biocompatibility, degradation rate, moisturizing effects, and stretchability of the developed hydrogels, as determined <jats:italic>in vitro</jats:italic>. Such multimaterial hydrogels can stimulate angiogenesis and exhibit desirable antibacterial properties. Therefore further (<jats:italic>in vivo</jats:italic>) tests are justified to assess the hydrogels’ potential for wound dressing and skin tissue healing applications.</jats:p>

Topics

• nanoparticle
• porous
• microstructure
• scanning electron microscopy
• glass
• glass
• composite
• biocompatibility
• X-ray spectroscopy
• aldehyde