| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Pinelo, Manuel
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (7/7 displayed)
- 2022Electrospun aluminum silicate nanofibers as novel support material for immobilization of alcohol dehydrogenasecitations
- 2022Electrospun aluminum silicate nanofibers as novel support material for immobilization of alcohol dehydrogenasecitations
- 2022Economic and environmental analysis of bio-succinic acid production: from established processes to a new continuous fermentation approach with in-situ electrolytic extractioncitations
- 2021Tailor-made novel electrospun polystyrene/poly(D,L-lactide-co-glycolide) for oxidoreductases immobilization: Improvement of catalytic properties under extreme reaction conditionscitations
- 2020Sustainable Process Synthesis, Design and Innovation of Bio-succinic Acid Productioncitations
- 2020The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removalcitations
- 2020Surface treatments and functionalization of metal‐ceramic membranes for improved enzyme immobilization performancecitations
Places of action
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article
Electrospun aluminum silicate nanofibers as novel support material for immobilization of alcohol dehydrogenase
Abstract
Ceramic materials with high surface area, large and open porosity are considered excellent supports for enzyme immobilization owing to their stability and reusability. The present study reports the electrospinning of aluminum silicate nanofiber supports from sol-gel precursors, the impact of different fabrication parameters on the microstructure of the nanofibers and their performance in enzyme immobilization. A change in nanofiber diameter and pore size of the alumina silicate nanofibers was observed upon varying specific processing parameters, such as the sol- composition (precursor and polymer concentration), the electrospinning parameters and the subsequent heat treatment (calcination temperature). The enzyme, alcohol dehydrogenase, was immobilized on the aluminum silicate nanofibers by physical adsorption and covalent bonding. Activity retention of 17 and 42 % was obtained after 12 days of storage and repeated reaction cycles for physically adsorbed and covalently bonded ADH, respectively. The immobilization of alcohol dehydrogenase on aluminum silicate nanofibers resulted in high enzyme loading and activity retention. However, a marked decrease in the enzyme activity during storage for physically adsorbed enzymes was observed, which was ascribed to leakage of the enzymes from the nanofibers. Such fibers are also able to improve enzyme stability and promote a higher residual activity of the immobilized enzyme as compared to the free enzyme. The results shown in this study thus suggest that aluminum silicate nanofibers, with their high surface area, are promising support materials for the immobilization of enzymes.