| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Hutmacher, D. W.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (17/17 displayed)
- 2020Human and mouse bones physiologically integrate in a humanized mouse model while maintaining species-specific ultrastructurecitations
- 2015BMP delivery complements the guiding effect of scaffold architecture without altering bone microstructure in critical-sized long bone defects: A multiscale analysiscitations
- 2015Additively Manufactured Device for Dynamic Culture of Large Arrays of 3D Tissue Engineered Constructscitations
- 20153D Tissue Growth in vivo under Geometrical Constraints
- 2014Advanced tissue engineering scaffold design for regeneration of the complex hierarchical periodontal structurecitations
- 2014Biofabrication of customized bone grafts by combination of additive manufacturing and bioreactor knowhowcitations
- 2012Porous scaffold architecture guides tissue formationcitations
- 2012All-in-one rapid-prototyped bioreactor/implant for semi-automated generation of tailor-made critical size bone tissue substitutes
- 2012Bone tissue engineering: From bench to bedsidecitations
- 2012A calcium phosphate coated biphasic scaffold for periodontal complex regeneration
- 2010Ceramic Materials for Bone Tissue Replacement and Regeneration
- 2009Composite PLDLLA/TCP Scaffolds for Bone Engineering: Mechanical and In Vitro Evaluations
- 2009Composite PLDLLA/TCP Scaffolds for Bone Engineeringcitations
- 2008Mechanical and in vitro evaluations of composite PLDLLA/TCP scaffolds for bone engineeringcitations
- 2006A comparative analysis of scaffold material modifications for load-bearing applications in bone tissue engineeringcitations
- 2003Osteogenic induction of human bone marrow-derived mesenchymal progenitor cells in novel synthetic polymer-hydrogel matricescitations
- 2002Preliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffoldscitations
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article
Mechanical and in vitro evaluations of composite PLDLLA/TCP scaffolds for bone engineering
Abstract
<p>Bone tissue engineering scaffolds have two challenging functional tasks to play; to be bioactive by encouraging cell proliferation and differentiation, and to provide suitable mechanical stability upon implantation. Composites of biopolymers and bioceramics unite the advantages of both materials, resulting in better processability, enhanced mechanical properties through matrix reinforcement and osteoinductivity. Novel composite blends of poly(L-lactide-co-D,L-lactide)/tricalcium phosphate (PLDLLA/TCP) were fabricated into scaffolds by an extrusion deposition technique customised from standard rapid prototyping technology. PLDLLA/TCP composite material blends of various compositions were prepared and analysed for their mechanical properties. PLDLLA/TCP (10%) was optimised and fabricated into scaffolds. Compressive mechanical properties for the composite scaffolds were measured. In vitro studies were conducted using porcine bone-marrow stromal cells (BMSCs). Cell-scaffold constructs were induced using osteogenic induction factors for up to 8 weeks. Cell proliferation, viability and differentiation capabilities were assayed using phase-contrast light microscopy, scanning electron microscopy, DNA quantification (Pico Green), Alamar Blue metabolic assay; FDA/PI fluorescent assay and western blot analysis for osteopontin. Microscopy observations showed BMSCs possessed high proliferative capabilities and demonstrated bridging across the pores of the scaffolds. FDA/PI staining as well as Alamar Blue assay showed high viability of BMSCs cultured on the composite scaffolds. Cell numbers, based on DNA quantitation, were observed to increase continuously up to the eighth week of study. Western blot analysis showed increased osteopontin synthesis on the scaffolds compared to tissue culture plastic. Based on our results the PLDLLA/TCP scaffolds exhibited good potential and biocompatibility for bone tissue engineering applications.</p>