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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Mergulhao, Fjm
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (6/6 displayed)
- 2022Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Reviewcitations
- 2021Assessment of the environmental compatibility and antifouling performance of an innovative biocidal and foul-release multifunctional marine coatingcitations
- 2021Developing New Marine Antifouling Surfaces: Learning from Single-Strain Laboratory Testscitations
- 2020Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approachcitations
- 2020Experimental Assessment of the Performance of Two Marine Coatings to Curb Biofilm Formation of Microfoulerscitations
- 2019Biofilm formation behaviour of marine filamentous cyanobacterial strains in controlled hydrodynamic conditionscitations
Places of action
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article
Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach
Abstract
Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s(-1)and 40 s(-1)). For both strains and surfaces, biofilm development was higher at 4 s(-1). Biofilm development ofNodosilineasp. LEGE 06145 was substantially higher thanNodosilineasp. LEGE 06119, but no significant differences were found between surfaces. Overall, 377 and 301 different proteins were identified forNodosilineasp. LEGE 06145 andNodosilineasp. LEGE 06119. Differences in protein composition were more noticeable in biofilms formed under different hydrodynamic conditions than in those formed on different surfaces. Ribosomal and photosynthetic proteins were identified in most conditions. The characterization performed gives new insights into how shear rate and surface affect the planktonic to biofilm transition, from a structural and proteomics perspective.