| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Gondran, Chantal
Université Grenoble Alpes
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (7/7 displayed)
- 2023Aptamer conjugated Si nanowire network/poly(pyrrole)-NTA structure for electrochemical sensingcitations
- 2021A cobalt oxide–polypyrrole nanocomposite as an efficient and stable electrode material for electrocatalytic water oxidationcitations
- 2020Voltammetric sensing of recombinant viral dengue virus 2 NS1 based on Au nanoparticle–decorated multiwalled carbon nanotube compositescitations
- 2010Contribution of iron clays in the preparation of amperometric biosensors : Study of the interaction of hemoglobin with Clays and Layered Doubles Hydroxides.
- 2008Electrochemical investigation of the influence of thin SiO x films deposited on gold on charge transfer characteristicscitations
- 2008Detection of carbohydrate-binding proteins by oligosaccharide-modified polypyrrole interfaces using electrochemical surface plasmon resonancecitations
- 2008Electrochemical investigation of the influence of thin SiO<sub><i>x</i></sub> films deposited on gold on charge transfer characteristicscitations
Places of action
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article
Detection of carbohydrate-binding proteins by oligosaccharide-modified polypyrrole interfaces using electrochemical surface plasmon resonance
Abstract
This paper reports on the use of electrochemical surface plasmon resonance (E-SPR) for the detection of carbohydrate-binding proteins. The generation of an SPR sensor specific to lectins <i>Arachis hypogaea</i> (PNA) and <i>Maackia amurensis</i> (MAA) is based on the electrochemical polymerization of oligosaccharide derivatives functionalized by pyrrole groups. The resulting thin conducting polymer films were characterized using E-SPR and atomic force microscopy (AFM). The specific binding of PNA to polypyrrole-lactosyl and of MAA to polypyrrole-3-sialyllactosyl films was investigated using SPR. The detection limit was 41 nM for PNA and 83 nM for MAA. Through Scatchard analysis and linear transformation of the SPR sensorgram data, association (<i>k</i><sub>ass</sub>) and dissociation rate constants (<i>k</i><sub>diss</sub>) could be determined.