| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Mayer, Philipp
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (15/15 displayed)
- 2024Determining ecotoxicity drivers and biodegradation kinetics of discharged chemicals in produced water from oil and gas extraction in the North Sea
- 2018Assessing PCB pollution in the Baltic Sea - An equilibrium partitioning based studycitations
- 2018Headspace passive dosing for dose-response testing of volatile hydrophobic organic chemicals
- 2015Comparison of passive and standard dosing of polycyclic aromatic hydrocarbons to the marine algae Phaeodactylum tricornutum
- 2015Equilibrium passive sampling as a tool to study polycyclic aromatic hydrocarbons in Baltic Sea sediment pore-water systemscitations
- 2014The effect of humic acids on biodegradation of polycyclic aromatic hydrocarbons depends on the exposure regimecitations
- 2013Baseline Toxic Mixtures of Non-Toxic Chemicalscitations
- 2013The dosing determines mutagenicity of hydrophobic compounds in the Ames II assay with metabolic transformationcitations
- 2012Recreating the seawater mixture composition of HOCs in toxicity tests with Artemia franciscana by passive dosingcitations
- 2011A Contaminant Trap as a Tool for Isolating and Measuring the Desorption Resistant Fraction of Soil Pollutantscitations
- 2011Application of passive dosing to study the biotransformation and biodegradation of hydrophobic compounds
- 2011Application of passive dosing to study the biotransformation and biodegradation of hydrophobic
- 2010Controlling and maintaining exposure of hydrophobic organic compounds in aquatic toxicity tests by passive dosingcitations
- 2010Passive Dosing for Producing Defined and Constant Exposure of Hydrophobic Organic Compounds during in Vitro Toxicity Testscitations
- 2009In Situ Silicone Tube Microextractioncitations
Places of action
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article
Passive Dosing for Producing Defined and Constant Exposure of Hydrophobic Organic Compounds during in Vitro Toxicity Tests
Abstract
Toxicity testing of hydrophobic organic compounds (HOCs') in plastic cell culture plates is problematic due to compound losses through volatilization and sorption to the wells and Culture medium constituents. This leads to poorly defined exposure and reduced test sensitivity. Passive dosing call overcome these problems by the continual partitioning of HOCs from a dominating reservoir loaded in a biologically inert polymer such as silicone, providing defined and constant freely dissolved concentrations and also eliminating spiking with cosolvents. This Study aimed to select a Suitable passive dosing format for in vitro tests ill multiwell plates and characterize its performance at 37 degrees C. Silicone O-rings were the most Suitable format; they were both practical and demonstrated excellent passive closing performance. (1) The rings were loaded by partitioning from a methanol solution containing polycyclic aromatic hydrocarbons (PAHs) (log K(OW), 3.33-6.43) that served as model compounds, followed by removal of the methanol with water. This resulted in highly reproducible HOC concentrations in the silicone O-rings. (2) The release of PAHs into aqueous solutions was rapid and reproducible, with equilibrium partitioning being reached within hours. (3) The buffering capacity of the O-rings was sufficient to maintain Stable concentrations over more than 72 h. The O-rings were then applied to test a range of PAHs at their aqueous Solubility in all array of established in vitro cell culture assays with human cells and cell lines. These included the formation of reactive oxygen species, induction of the IL-8 cytokine promoter, and secretion of MCF-1 by the cells. The biological responses depended oil the melting point of the individual PAHs and their maximum chemical activities (a(max)), Only those PAHs with the highest a(max) stimulated the formation of reactive oxygen species and MCP-1 secretion, while they inhibited the induction of the IL-8 cytokine promoter.