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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Madeira, Pp
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (6/6 displayed)
- 2013Analysis of amino acid-water interactions by partitioning in aqueous two-phase systems. I-Amino acids with non-polar side-chainscitations
- 2008Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systemscitations
- 2008"On the Collander equation": Protein partitioning in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) in PEG-salt and Ucon-salt aqueous two-phase systemscitations
- 2004Representation of liquid-liquid equilibria for polymer-salt aqueous two-phase systemscitations
Places of action
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article
Delta G(CH2) in PEG-salt and Ucon-salt aqueous two-phase systems
Abstract
Three new phase diagrams for polymer-salt aqueous two-phase systems (ATPS) at 23 degrees C are presented. Then, partition coefficients for a series of five dinitrophenylated amino acids were obtained experimentally in five polymer-salt ATPS. The ATPS were obtained combining a polymer, polyethylene glycol (PEG) or Ucon (a random copolymer of 50 % ethylene oxide and 50 % propylene oxide), and a salt (Na2SO4, (NH4)(2)SO4, or Li2SO4). The series of amino acids allowed the calculation of the free energy of transfer of a methylene group, which is used as a measure of the relative hydrophobicity of the equilibrium phases. Ucon-rich phases proved to have a higher affinity for methylene groups than PEG which suggests a more efficient biphasic separation system with lower cross-contamination.