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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Macedo, Ea
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Topics
Publications (8/8 displayed)
- 2024Liquid-Liquid Equilibria Determination and Modeling of ATPS Based on PEG4000 and PVP29000 at 298.15 K and 0.1 MPacitations
- 2012(Liquid plus liquid) equilibria of polymer-salt aqueous two-phase systems for laccase partitioning: UCON 50-HB-5100 with potassium citrate and (sodium or potassium) formate at 23 degrees Ccitations
- 2010Liquid-Liquid Equilibria of UCON plus (Sodium or Potassium) Phosphate Salt Aqueous Two-Phase Systems at 23 degrees Ccitations
- 2008Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systemscitations
- 2008"On the Collander equation": Protein partitioning in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) in PEG-salt and Ucon-salt aqueous two-phase systemscitations
- 2004Representation of liquid-liquid equilibria for polymer-salt aqueous two-phase systemscitations
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article
Delta G(CH2) in PEG-salt and Ucon-salt aqueous two-phase systems
Abstract
Three new phase diagrams for polymer-salt aqueous two-phase systems (ATPS) at 23 degrees C are presented. Then, partition coefficients for a series of five dinitrophenylated amino acids were obtained experimentally in five polymer-salt ATPS. The ATPS were obtained combining a polymer, polyethylene glycol (PEG) or Ucon (a random copolymer of 50 % ethylene oxide and 50 % propylene oxide), and a salt (Na2SO4, (NH4)(2)SO4, or Li2SO4). The series of amino acids allowed the calculation of the free energy of transfer of a methylene group, which is used as a measure of the relative hydrophobicity of the equilibrium phases. Ucon-rich phases proved to have a higher affinity for methylene groups than PEG which suggests a more efficient biphasic separation system with lower cross-contamination.