| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Chabbert, Brigitte
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (22/22 displayed)
- 2023Influence of surface chemistry of fiber and lignocellulosic materials on adhesion properties with polybutylene succinate at nanoscalecitations
- 2023Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccasecitations
- 2023Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccasecitations
- 2023Influence of Surface Chemistry of Fiber and Lignocellulosic Materials on Adhesion Properties with Polybutylene Succinate at Nanoscalecitations
- 2023Influence of Surface Chemistry of Fiber and Lignocellulosic Materials on Adhesion Properties with Polybutylene Succinate at Nanoscalecitations
- 2020Multiscale modeling of microbial degradation of outer tissues of fiber-crop stems during the dew retting processcitations
- 2020Influence of the polarity of the matrix on the breakage mechanisms of lignocellulosic fibers during twin-screw extrusioncitations
- 2020Influence of the polarity of the matrix on the breakage mechanisms of lignocellulosic fibers during twin-screw extrusioncitations
- 2020Atomic force microscopy reveals how relative humidity impacts the Young’s modulus of lignocellulosic polymers and their adhesion with cellulose nanocrystals at the nanoscalecitations
- 2020Effect of the Interplay of Composition and Environmental Humidity on the Nanomechanical Properties of Hemp Fiberscitations
- 2020Multimodal assessment of flax dew retting and its functional impact on fibers and natural fiber compositescitations
- 2015Nanocharacterization of chemical and mechanical properties of plant cell walls and lignocellulosic bioinspired assemblies
- 2015Nanocharacterization of chemical and mechanical properties of plant cell walls and lignocellulosic bioinspired assemblies
- 2015Fungal elicitor-mediated enhancement in phenylpropanoid and naphtodianthrone contents of Hypericum perforatum L. cell culturescitations
- 2015Use of food and packaging model Matrices to investigate the antioxidant properties of biorefinery grass ligninscitations
- 2015Organosolv lignin as natural grafting additive to improve the water resistance of films using cellulose nanocrystalscitations
- 2014Atomic Force Microscopy-related techniques applied to the nanocharacterization of mechanical and chemical properties of plant cell walls and bioinspired polymer films
- 2012Natural Organic UV-Absorbent Coatings Based on Cellulose and Lignin: Designed Effects on Spectroscopic Propertiescitations
- 2012Effect of lignin content on a GH11 endoxylanase acting on glucuronoarabinoxylan-lignin nanocompositescitations
- 2011Structure and optical properties of plant cell wall bioinspired materials: cellulose-lignin multilayer nanocompositescitations
- 2009In vitro model assemblies to study the impact of lignin-carbohydrate interactions on the enzymatic conversion of Xylancitations
- 2009In vitro model assemblies to study the impact of lignin-carbohydrate interactions on the enzymatic conversion of Xylancitations
Places of action
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article
In vitro model assemblies to study the impact of lignin-carbohydrate interactions on the enzymatic conversion of Xylan
Abstract
Endo-beta-1,4-xylanases (EC 3.2.1.8) are the main enzymes involved in the hydrolysis of xylans, the most abundant hemicelluloses in plant. biomass. However, the development of efficient endoxylanases for use in biorefinery processes is currently hampered by insufficient knowledge regarding the impact of the cell wall network organization on the action of the enzyme at the supramolecular level. The action pattern of a GH11 endoxylanase from Thermobacillus xylanilyticus (Tx-xyl) was investigated by means of in vitro reconstituted model systems which can mimic certain cell wall structures. The action of Tx-xyl was evaluated on polymer assemblies displaying increasing complexity using delignified glucuronoarabinoxylan (GAX), then GAX-DHP model complexes obtained by oxidative polymerization of coniferyl alcohol into dehydrogenation polymers (DHP: lignin model compounds) in the presence of GAX At a high concentration of GAX, interchain associations are formed leading to high molecular weight aggregates. These structures did not appear to affect the action of endoxylanase, which induces disaggregation of the self-aggregates along with polymer depolymerization. To mimic lignin-carbohydrate interactions, two different GAX-DHP nanocomposites were prepared and incubated with endoxylanase. In both cases, free GAX was hydrolyzed, while the GAX-DHP complexes appeared to be resistant. In the case of the noncovalently linked GAX-DHP(ZL) complexes, enzyme action favored a decrease in particle size, owing to the removal of their relatively exposed carbohydrate chains, whereas the complex supramolecular organization of the covalently linked GAX-DHP(ZT) complexes severely hampers the enzyme's access to carbohydrate. Overall, these results establish the negative impact of DHP on the endoxylanase action and provide new knowledge regarding the limitations of the enzyme action in the lignocellulose bioconversion processes.