| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Nogués, C.
Universitat Autònoma de Barcelona
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (17/17 displayed)
- 2023Accelerated biodegradation of FeMn porous alloy coated with ZnOcitations
- 2023Surface Modified β-Ti-18Mo-6Nb-5Ta (wt%) Alloy for Bone Implant Applications:citations
- 2023Hierarchical Surface Pattern on Ni‐Free Ti‐Based Bulk Metallic Glass to Control Cell Interactionscitations
- 2022Biodegradable porous FeMn(-xAg) alloys:citations
- 2018Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness, and enhanced osteoblast differentiation for biomedical applications
- 2017Study of Galfenol direct cytotoxicity and remote microactuation in cellscitations
- 2017Mechanical properties, corrosion performance and cell viability studies on newly developed porous Fe-Mn-Si-Pd alloyscitations
- 2016Effect of surface modifications of Ti40Zr10Cu38Pd12 bulk metallic glass and Ti-6Al-4V alloy on human osteoblasts in vitro biocompatibilitycitations
- 2016Novel Fe-Mn-Si-Pd alloys: Insights into mechanical, magnetic, corrosion resistance and biocompatibility performancescitations
- 2015Nanostructured Ti-Zr-Pd-Si-(Nb) bulk metallic composites: Novel biocompatible materials with superior mechanical strength and elastic recoverycitations
- 2015Controlling colloidal stability of silica nanoparticles during bioconjugation reactions with proteins and improving their longer-term stability, handling and storagecitations
- 2014In vitro biocompatibility assessment of Ti40Cu38Zr10Pd12 bulk metallic glasscitations
- 2014Optimized immobilization of lectins using self-assembled monolayers on polysilicon encoded materials for cell taggingcitations
- 2013On the biodegradability, mechanical behavior, and cytocompatibility of amorphous Mg72Zn23Ca5 and crystalline Mg70Zn23Ca5Pd2 alloys as temporary implant materialscitations
- 2013Novel Ti-Zr-Hf-Fe nanostructured alloy for biomedical applicationscitations
- 2012Efficient biofunctionalization of polysilicon barcodes for adhesion to the zona pellucida of mouse embryoscitations
- 2012Improved mechanical performance and delayed corrosion phenomena in biodegradable Mg-Zn-Ca alloys through Pd-alloyingcitations
Places of action
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article
Efficient biofunctionalization of polysilicon barcodes for adhesion to the zona pellucida of mouse embryos
Abstract
Cell tracking is an emergent area in nanobiotechnology, promising the study of individual cells or the identification of populations of cultured cells. In our approach, microtools designed for extracellular tagging are prepared, because using biofunctionalized polysilicon barcodes to tag cell membranes externally avoids the inconveniences of cell internalization. The crucial covalent biofunctionalization process determining the ultimate functionality was studied in order to find the optimum conditions to link a biomolecule to a polysilicon barcode surface using a self-assembled monolayer (SAM) as the connector. Specifically, a lectin (wheat germ agglutinin, WGA) was used because of its capacity to recognize some specific carbohydrates present on the surface of most mammalian cells. Self-assembled monolayers were prepared on polysilicon surfaces including aldehyde groups as terminal functions to study the suitability of their covalent chemical bonding to WGA. Some parameters, such as the polysilicon surface roughness or the concentration of WGA, proved to be crucial for successful biofunctionalization and bioactivity. The SAMs were characterized by contact angle measurements, time-of-flight secondary ion mass spectrometry (TOF-SIMS), laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS), and atomic force microscopy (AFM). The biofunctionalization step was also characterized by fluorescence microscopy and, in the case of barcodes, by adhesion experiments to the zona pellucida of mouse embryos. These experiments showed high barcode retention rates after 96 h of culture as well as high embryo viability to the blastocyst stage, indicating the robustness of the biofunctionalization and, therefore, the potential of these new microtools to be used for cell tagging. © 2012 American Chemical Society.