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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Song, W.
Engineering and Physical Sciences Research Council
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2022Nano-sized Cu precipitation and microstructural evolution in aged ultralow and medium carbon steelscitations
- 2021<i>Komagataeibacter</i> Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria.citations
- 2020Bio-inspired multi-resonant acoustic devices based on electrospun piezoelectric polymeric nanofibres
- 2019Thermoresponsive Stiffness Softening of Hierarchically Porous Nanohybrid Membranes Promotes Niches for Mesenchymal Stem Cell Differentiation
- 2018Translucent, color-neutral and efficient perovskite thin film solar modules:
- 2018Carbon redistribution in martensite in high-C steel: Atomic-scale characterization and modelling
- 2018Co₃O₄ hollow nanospheres doped with ZnCo₂O₄ via thermal vapor mechanism for fast lithium storage
- 2017Enhancing Distorted Metal-Organic Framework-Derived ZnO as Anode Material for Lithium Storage by the Addition of Ag2S Quantum Dots
- 2017Enhancing Distorted Metal Organic Framework Derived ZnO as Anode Material for Lithium Storage by the Addition of Ag2S Quantum Dots.citations
- 2013Atomic-scale investigation of epsilon and theta precipitates in bainite in 100Cr6 bearing steel by atom probe tomography and ab initio calculationscitations
- 2012Wettability influences cell behavior on superhydrophobic surfaces with different topographies.
- 2012Wettability influences cell behavior on superhydrophobic surfaces with different topographiescitations
- 2010Effect of temperature and role of Mo top layer on the growth of carbon nanotubescitations
Places of action
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article
<i>Komagataeibacter</i> Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria.
Abstract
Bacteria proficient at producing cellulose are an attractive synthetic biology host for the emerging field of Engineered Living Materials (ELMs). Species from the <i>Komagataeibacter</i> genus produce high yields of pure cellulose materials in a short time with minimal resources, and pioneering work has shown that genetic engineering in these strains is possible and can be used to modify the material and its production. To accelerate synthetic biology progress in these bacteria, we introduce here the <i>Komagataeibacter</i> tool kit (KTK), a standardized modular cloning system based on Golden Gate DNA assembly that allows DNA parts to be combined to build complex multigene constructs expressed in bacteria from plasmids. Working in <i>Komagataeibacter rhaeticus</i>, we describe basic parts for this system, including promoters, fusion tags, and reporter proteins, before showcasing how the assembly system enables more complex designs. Specifically, we use KTK cloning to reformat the <i>Escherichia coli</i> curli amyloid fiber system for functional expression in <i>K. rhaeticus</i>, and go on to modify it as a system for programming protein secretion from the cellulose producing bacteria. With this toolkit, we aim to accelerate modular synthetic biology in these bacteria, and enable more rapid progress in the emerging ELMs community.