| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Lee, Koon-Yang
Imperial College London
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (23/23 displayed)
- 2023Predicting filling efficiency of composite resin injection repaircitations
- 2021<i>Komagataeibacter</i> Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria.citations
- 2020Nanomaterials Derived from Fungal Sources-Is It the New Hype?citations
- 2020Upcycling Poultry Feathers with (Nano)cellulose:Sustainable Composites Derived from Nonwoven Whole Feather Preformscitations
- 2020High porosity cellulose nanopapers as reinforcement in multi-layer epoxy laminatescitations
- 2019Nanocellulose reinforced polymer composites: Computational analysis of structure-mechanical properties relationshipscitations
- 2019Cationic starch as strengthening agent in nanofibrillated and bacterial cellulose nanopapers
- 2019Nanomaterials Derived from Fungal Sources - Is It the New Hype?citations
- 2018Better togethercitations
- 2018Thinner and better: (Ultra-)low grammage bacterial cellulose nanopaper-reinforced polylactide composite laminates
- 2017Sample geometry dependency on the measured tensile properties of cellulose nanopaperscitations
- 2016Understanding the Dispersion and Assembly of Bacterial Cellulose in Organic Solventscitations
- 2016Ductile unidirectional continuous rayon fibre-reinforced hierarchical compositescitations
- 2014Bacterial Cellulose Nanopaper as Reinforcement for Polylactide Compositescitations
- 2014Aligned unidirectional PLA/bacterial cellulose nanocomposite fibre reinforced PDLLA compositescitations
- 2014On the use of nanocellulose as reinforcement in polymer matrix compositescitations
- 2013Porous copolymers of ε-caprolactone as scaffolds for tissue engineeringcitations
- 2012Nano-fibrillated cellulose vs bacterial cellulose
- 2012Carbon Fiber: Properties, Testing, and Analysiscitations
- 2012Interfaces in Cross-Linked and Grafted Bacterial Cellulose/Poly(Lactic Acid) Resin Compositescitations
- 2012Nano-fibrillated cellulose vs bacterial cellulose:Reinforcing ability of nanocellulose obtained topdown or bottom-up
- 2009Renewable nanocomposite polymer foams synthesized from Pickering emulsion templatescitations
- 2009Surface functionalisation of bacterial cellulose as the route to produce green polylactide nanocomposites with improved propertiescitations
Places of action
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article
<i>Komagataeibacter</i> Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria.
Abstract
Bacteria proficient at producing cellulose are an attractive synthetic biology host for the emerging field of Engineered Living Materials (ELMs). Species from the <i>Komagataeibacter</i> genus produce high yields of pure cellulose materials in a short time with minimal resources, and pioneering work has shown that genetic engineering in these strains is possible and can be used to modify the material and its production. To accelerate synthetic biology progress in these bacteria, we introduce here the <i>Komagataeibacter</i> tool kit (KTK), a standardized modular cloning system based on Golden Gate DNA assembly that allows DNA parts to be combined to build complex multigene constructs expressed in bacteria from plasmids. Working in <i>Komagataeibacter rhaeticus</i>, we describe basic parts for this system, including promoters, fusion tags, and reporter proteins, before showcasing how the assembly system enables more complex designs. Specifically, we use KTK cloning to reformat the <i>Escherichia coli</i> curli amyloid fiber system for functional expression in <i>K. rhaeticus</i>, and go on to modify it as a system for programming protein secretion from the cellulose producing bacteria. With this toolkit, we aim to accelerate modular synthetic biology in these bacteria, and enable more rapid progress in the emerging ELMs community.