| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Landini, Gabriel
University of Birmingham
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (15/15 displayed)
- 2023Multi-resolution Correlative Ultrastructural and Chemical Analysis of Carious Enamel by Scanning Microscopy and Tomographic Imagingcitations
- 2018Automated non-invasive cell counting in phase contrast microscopy with automated image analysis parameter selectioncitations
- 2018Structure-function correlative microscopy of peritubular and intertubular dentinecitations
- 2017Model-based Correction of Segmentation Errors in Digitised Histological Imagescitations
- 2016Understanding nature’s residual strain engineering at the human dentine-enamel junction interfacecitations
- 2015Automated optimisation of cell segmentation parameters in phase contrast using discrete mereotopology
- 2014Structure-mechanical function relations at nano-scale in heat-affected human dental tissuecitations
- 2014Hierarchical modelling of in situ elastic deformation of human enamel based on photoelastic and diffraction analysis of stresses and strainscitations
- 2014Semi-automated cell counting in phase contrast images of epithelial monolayers
- 2014Nano-scale thermo-mechanical structure-property relationships in human dental tissues studied by nanoindentation and synchrotron X-ray scatteringcitations
- 2013Hierarchical modelling of elastic behaviour of human enamel based on synchrotron diffraction characterisationcitations
- 2013Multiscale modelling and diffraction-based characterization of elastic behaviour of human dentinecitations
- 2009Ultrasonic Scaler Oscillations and Tooth-surface Defectscitations
- 2007Influence of compressive and deflective force on powered toothbrush filaments
- 2001The influence of mixing ratio on the toughening mechanisms of a hand-mixed zinc phosphate dental cementcitations
Places of action
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article
Multi-resolution Correlative Ultrastructural and Chemical Analysis of Carious Enamel by Scanning Microscopy and Tomographic Imaging
Abstract
Caries, a major global disease associated with dental enamel demineralization, remains insufficiently understood to devise effective prevention or minimally invasive treatment. Understanding the ultrastructural changes in enamel is hampered by a lack of nanoscale characterization of the chemical spatial distributions within the dental tissue. This leads to the requirement to develop techniques based on various characterization methods. The purpose of the present study is to demonstrate the strength of analytic methods using a correlative technique on a single sample of human dental enamel as a specific case study to test the accuracy of techniques to compare regions in enamel. The science of the different techniques is integrated to genuinely study the enamel. The hierarchical structures within carious tissue were mapped using the combination of focused ion beam scanning electron microscopy with synchrotron X-ray tomography. The chemical changes were studied using scanning X-ray fluorescence (XRF) and X-ray wide-angle and small-angle scattering using a beam size below 80 nm for ångström and nanometer length scales. The analysis of XRF intensity gradients revealed subtle variations of Ca intensity in carious samples in comparison with those of normal mature enamel. In addition, the pathways for enamel rod demineralization were studied using X-ray ptychography. The results show the chemical and structural modification in carious enamel with differing locations. These results reinforce the need for multi-modal approaches to nanoscale analysis in complex hierarchically structured materials to interpret the changes of materials. The approach establishes a meticulous correlative characterization platform for the analysis of biomineralized tissues at the nanoscale, which adds confidence in the interpretation of the results and time-saving imaging techniques. The protocol demonstrated here using the dental tissue sample can be applied to other samples for statistical study and the investigation of nanoscale structural changes. The information gathered from the combination of methods could not be obtained with traditional individual techniques.