| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Taboryski, Rafael Jozef
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (34/34 displayed)
- 20223D printed microrobots controlled by light – Towards environmental and biomedical applications
- 2021Micro 3D Printing by Two-Photon Polymerization: Configurations and Parameters for the Nanoscribe Systemcitations
- 2021Bioinspired microstructured polymer surfaces with antireflective propertiescitations
- 2021Photolithographic Patterning of FluorAcryl for Biphilic Microwell-Based Digital Bioassays and Selection of Bacteriacitations
- 2021Influence of Thermo-Mechanical Mismatch when Nanoimprinting Anti-Reflective Structures onto Small-core Mid-IR Chalcogenide Fibers
- 2021Thermo-mechanical dynamics of nanoimprinting anti-reflective structures onto small-core mid-IR chalcogenide fiberscitations
- 2021Additive manufacturing of polymeric scaffolds for biomimetic cell membrane engineeringcitations
- 2020Engineering of wetting properties for solid surfaces
- 2019Nanoimprinting reflow modified moth-eye structures in chalcogenide glass for enhanced broadband antireflection in the mid-infraredcitations
- 2018Direct nanoimprinting of moth-eye structures in chalcogenide glass for broadband antireflection in the mid-infraredcitations
- 2018Transition to Superwetting for a Nanostructured Surface
- 2018Mapping the transition to superwetting state for nanotextured surfaces templated from block-copolymer self-assemblycitations
- 2016Free-form nanostructured tools for plastic injection moulding
- 2016Laser ablated micropillar energy directors for ultrasonic welding of microfluidic systemscitations
- 2016Determination of stamp deformation during imprinting on semi-spherical surfaces
- 2016Replication of nanopits and nanopillars by roll-to-roll extrusion coating using a structured cooling rollcitations
- 2016MICRO-SCALE ENERGY DIRECTORS FOR ULTRASONIC WELDING
- 2016Rapid Voltammetric Measurements at Conducting Polymer Microelectrodes Using Ultralow-Capacitance Poly(3,4-ethylenedioxythiophene):Tosylatecitations
- 2016Comparison of Ultrasonic Welding and Thermal Bonding for the Integration of Thin Film Metal Electrodes in Injection Molded Polymeric Lab-on-Chip Systems for Electrochemistrycitations
- 2015Multi-height structures in injection molded polymercitations
- 2015Smart plastic functionalization by nanoimprint and injection moldingcitations
- 2015Fiber-Based, Injection-Molded Optofluidic Systemscitations
- 2014Characterization of Injection Molded Structures
- 2014Fabrication and modelling of injection moulded all-polymer capillary microvalves for passive microfluidic controlcitations
- 2013A method for manufacturing a tool part for an injection molding process, a hot embossing process, a nano-imprint process, or an extrusion process
- 2013Ion channel recordings on an injection-molded polymer chipcitations
- 2013Fabrication and characterization of injection molded multi level nano and microfluidic systemscitations
- 2013Surface roughness reduction using spray-coated hydrogen silsesquioxane reflowcitations
- 2012A Pressure Controlled Pinched Flow Fractionation Device for Continuous Particle Separation
- 2012Evaluation of stability for monolayer injection molding tools coating
- 2012Stability of FDTS monolayer coating on aluminum injection molding toolscitations
- 2012All polymer, injection molded nanoslits, fabricated through two-level UV-LIGA processes
- 2012Fabrication of combined-scale nano- and microfluidic polymer systems using a multilevel dry etching, electroplating and molding processcitations
- 2010Oxygen Barrier Coating Deposited by Novel Plasma-enhanced Chemical Vapor Depositioncitations
Places of action
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article
Photolithographic Patterning of FluorAcryl for Biphilic Microwell-Based Digital Bioassays and Selection of Bacteria
Abstract
FluorAcryl 3298 (FA) is a UV-curable fluoroacrylate polymer commonly employed as a chemically resistant, hydrophobic, and oleophobic coating. Here, FA was used in a cleanroom-based microstructuring process to fabricate hydrophilic-in-hydrophobic (HiH) micropatterned surfaces containing femtoliter-sized well arrays. A short protocol involving direct UV photopatterning, an etching step, and final recovery of the hydrophobic properties of the polymer produced patterned substrates with micrometer resolution. Specifically, HiH microwell arrays were obtained with a well diameter of 10 μm and various well depths ranging from 300 nm to 1 μm with high reproducibility. The 300 nm deep microdroplet array (MDA) substrates were used for digital immunoassays, which presented a limit of detection in the attomolar range. This demonstrated the chemical functionality of the hydrophilic and hydrophobic surfaces. Furthermore, the 1 μm deep wells could efficiently capture particles such as bacteria, whereas the 300 nm deep substrates or other types of flat HiH molecular monolayers could not. Capturing a mixture of bacteria expressing red- and green-fluorescent proteins, respectively, served as a model for screening and selection of specific phenotypes using FA-MDAs. Here, green-fluorescent bacteria were specifically selected by overlaying a solution of gelatin methacryloyl (GelMA) mixed with a photoinitiator and using a high-magnification objective, together with custom pinholes, in a common fluorescence microscope to cross-link the hydrogel around the bacteria of interest. In conclusion, due to the straightforward processing, versatility, and low-price, FA is an advantageous alternative to more commonly used fluorinated materials, such as CYTOP or Teflon-AF, for the fabrication of HiH microwell arrays and other biphilic microstructures.