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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Shi, Y.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (26/26 displayed)
- 2024Reactive passivation of wide-bandgap organic–inorganic perovskites with benzylaminecitations
- 2024Improved reverse bias stability in p–i–n perovskite solar cells with optimized hole transport materials and less reactive electrodescitations
- 2023Statistical evaluation of the mechanical and flux trapping properties of standard and thin-wall EuBCO(Ag) bulk superconductors
- 2023Tunable unconventional kagome superconductivity in charge ordered RbV3Sb5 and KV3Sb5citations
- 2022The processing and properties of bulk (RE)BCO high temperature superconductors: Current status and future perspectives
- 2022Identification and mechanistic basis of non-ACE2 blocking neutralizing antibodies from COVID-19 patients with deep RNA sequencing and molecular dynamics simulations.citations
- 2022A cracking oxygen story: a new view of stress corrosion cracking in titanium alloyscitations
- 2021AgCl-induced hot salt stress corrosion cracking in a titanium alloycitations
- 2021Roughening improves hydrogen embrittlement resistance of Ti-6Al-4Vcitations
- 2021A reliable technique to fabricate superconducting joints between single grain, Y–Ba–Cu–O bulk superconductors
- 2021<i>In Vitro</i> Development of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds.citations
- 2020Impact damage characteristics of carbon fibre metal laminates : experiments and simulation
- 2020Impact Damage Characteristics of Carbon Fibre Metal Laminates: Experiments and Simulationcitations
- 2020Two-photon laser-written photoalignment layers for patterning liquid crystalline conjugated polymer orientationcitations
- 2020Composite stacks for reliable > 17 T trapped fields in bulk superconductor magnets
- 2020Characterisation of the mechanical failure and fracture mechanisms of single grain Y-Ba-Cu-O bulk superconductors
- 2019Characterization of hardness and stiffness of ceramic matrix composites through instrumented indentation testcitations
- 2018A phase field method for modeling anodic dissolution induced stress corrosion crack propagationcitations
- 2017Improvements in the processing of large grain, bulk Y–Ba–Cu–O superconductors via the use of additional liquid phase
- 2017Multiple Cracks Interactions in Stress Corrosion Cracking: In Situ Observation by Digital Image Correlation and Phase Field Modelingcitations
- 2016The use of buffer pellets to pseudo hot seed (RE)-Ba-Cu-O-(Ag) single grain bulk superconductors
- 2016Role of Associated Defects in Oxygen Ion Conduction and Surface Exchange Reaction for Epitaxial Samaria-Doped Ceria Thin Films as Catalytic Coatingscitations
- 2014Interface cohesive elements to model matrix crack evolution in composite laminatescitations
- 2014Modelling impact damage in composite laminates: A simulation of intra- and inter-laminar crackingcitations
- 2012Modelling damage evolution in composite laminates subjected to low velocity impactcitations
- 20127.3 Gbit/s transmission over microstructured polymer optical fiber for in-home networkscitations
Places of action
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article
<i>In Vitro</i> Development of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds.
Abstract
Neural progenitor cells generated from human induced pluripotent stem cells (hiPSCs) are the forefront of ″brain-on-chip″ investigations. Viable and functional hiPSC-derived neuronal networks are shaping powerful <i>in vitro</i> models for evaluating the normal and abnormal formation of cortical circuits, understanding the underlying disease mechanisms, and investigating the response to drugs. They therefore represent a desirable instrument for both the scientific community and the pharmacological industry. However, culture conditions required for the full functional maturation of individual neurons and networks are still unidentified. It has been recognized that three-dimensional (3D) culture conditions can better emulate <i>in vivo</i> neuronal tissue development compared to 2D cultures and thus provide a more desirable <i>in vitro</i> approach. In this paper, we present the design and implementation of a 3D scaffold platform that supports and promotes intricate neuronal network development. 3D scaffolds were produced through direct laser writing by two-photon polymerization (2PP), a high-resolution 3D laser microstructuring technology, using the biocompatible and nondegradable photoreactive resin Dental LT Clear (DClear). Neurons developed and interconnected on a 3D environment shaped by vertically stacked scaffold layers. The developed networks could support different cell types. Starting at the day 50 of 3D culture, neuronal progenitor cells could develop into cortical projection neurons (CNPs) of all six layers, different types of inhibitory neurons, and glia. Additionally and in contrast to 2D conditions, 3D scaffolds supported the long-term culturing of neuronal networks over the course of 120 days. Network health and functionality were probed through calcium imaging, which revealed a strong spontaneous neuronal activity that combined individual and collective events. Taken together, our results highlight advanced microstructured 3D scaffolds as a reliable platform for the 3D <i>in vitro</i> modeling of neuronal functions.