• Arlinghaus, Henrich F.
• Lamping, Sebastian
• Korsgen, Martin
• Buten, Christoph
• Jamieson, Craig
• Ravoo, Bart Jan

Abstract

In this paper, we show that carboxylic acid-functionalized molecules can be patterned by photochemical microcontact printing on tetrazole-terminated self-assembled monolayers. Upon irradiation, tetrazoles eliminate nitrogen to form highly reactive nitrile imines, which can be ligated with several different nucleophiles, carboxylic acids being the most reactive. As a proof of concept, we immobilized trifluoroacetic acid to monitor the reaction with X-ray photoelectron spectroscopy. Moreover, we also immobilized peptides and fabricated carbohydrate−lectin as well as biotin−streptavidin microarrays using this method. Surface-patterning was demonstrated by fluorescence microscopy and time-of-flight secondary ion mass spectrometry

Topics

• surface
• x-ray photoelectron spectroscopy
• reactive
• Nitrogen
• functionalization
• spectrometry
• secondary ion mass spectrometry
• fluorescence microscopy
• carboxylic acid
• nitrile