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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Isoaho, Noora
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (6/6 displayed)
- 2022Microfabrication atomic layer deposited Pt NPs/TiN thin film on silicon as a nanostructure signal Transducer: Electrochemical characterization toward neurotransmitter sensingcitations
- 2019Integrating Carbon Nanomaterials with Metals for Bio-sensing Applicationscitations
- 2018Carbon Nanostructures for Enzymatic Electrochemical Biosensors ; Hiilinanorakenteet entsymaattisten sähkökemiallisten bioanturien valmistamiseencitations
- 2017Amorphous carbon thin film electrodes with intrinsic Pt-gradient for hydrogen peroxide detectioncitations
- 2017Amorphous carbon thin film electrodes with intrinsic Pt-gradient for hydrogen peroxide detectioncitations
- 2017Carbon Nanostructure Based Platform for Enzymatic Glutamate Biosensorscitations
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article
Carbon Nanostructure Based Platform for Enzymatic Glutamate Biosensors
Abstract
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is an important molecule produced in various enzymatic reactions. It is especially important in electrochemical, enzymatic biosensors detecting electroinactive analytes, such as glucose, cholesterol, and glutamate. Thus, there is a strong need for materials that have high affinity for H<sub>2</sub>O<sub>2</sub> oxidation or reduction as well as enable immobilization and sustain enzyme activity without any additional polymer layers. Carbon nanofibers (CNFs) directly grown on tetrahedral amorphous carbon (ta-C) are feasible candidates for this purpose as they possess a reasonably wide water window (1.8 V) and good activity for H<sub>2</sub>O<sub>2</sub> reduction in physiological pH and contain innately large amounts of suitable functional groups for enzyme immobilization. Here we show their use in ultrafast (<0.05 s) detection of H<sub>2</sub>O<sub>2</sub> with the limit of detection of 26 μM and sensitivity of 0.221 A M<sup>–1</sup> cm<sup>–2</sup>. Moreover, we show that ta-C/CNF hybrids can be used directly without the mass-transfer limiting polymer layers as effective immobilization platforms for glutamate oxidase for further applications in ultrafast (<0.05 s) glutamate detection. Finally, rat glial cells cultured on CNFs grown from ta-C without any additional coatings, such as polylysine, showed good adhesion on CNFs and no signs of cytotoxicity, indicating suitability of the material for future in vivo applications. This simplified and miniaturized structure provides an extremely interesting platform for various different enzyme-based electrochemical sensors.