| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Peltola, Emilia
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2023Interface matters - Effects of catalyst layer metallurgy on macroscale morphology and electrochemical performance of carbon nanofiber electrodescitations
- 2022Nanoscale geometry determines mechanical biocompatibility of vertically aligned nanofiberscitations
- 2021Nanostructured Geometries Strongly Affect Fouling of Carbon Electrodescitations
- 2020Biofouling affects the redox kinetics of outer and inner sphere probes on carbon surfaces drastically differently - implications to biosensingcitations
- 2019Integrating Carbon Nanomaterials with Metals for Bio-sensing Applicationscitations
- 2019Fabrication of micro- and nanopillars from pyrolytic carbon and tetrahedral amorphous carboncitations
- 2018Unmodified and multi-walled carbon nanotube modified tetrahedral amorphous carbon (ta-C) films as in vivo sensor materials for sensitive and selective detection of dopaminecitations
- 2018Nanodiamonds on tetrahedral amorphous carbon significantly enhance dopamine detection and cell viabilitycitations
- 2017Amorphous carbon thin film electrodes with intrinsic Pt-gradient for hydrogen peroxide detectioncitations
- 2017Amorphous carbon thin film electrodes with intrinsic Pt-gradient for hydrogen peroxide detectioncitations
- 2017Carbon Nanostructure Based Platform for Enzymatic Glutamate Biosensorscitations
- 2017Nanodiamonds on tetrahedral amorphous carbon significantly enhance dopamine detection and cell viabilitycitations
- 2015Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling propertiescitations
Places of action
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article
Carbon Nanostructure Based Platform for Enzymatic Glutamate Biosensors
Abstract
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is an important molecule produced in various enzymatic reactions. It is especially important in electrochemical, enzymatic biosensors detecting electroinactive analytes, such as glucose, cholesterol, and glutamate. Thus, there is a strong need for materials that have high affinity for H<sub>2</sub>O<sub>2</sub> oxidation or reduction as well as enable immobilization and sustain enzyme activity without any additional polymer layers. Carbon nanofibers (CNFs) directly grown on tetrahedral amorphous carbon (ta-C) are feasible candidates for this purpose as they possess a reasonably wide water window (1.8 V) and good activity for H<sub>2</sub>O<sub>2</sub> reduction in physiological pH and contain innately large amounts of suitable functional groups for enzyme immobilization. Here we show their use in ultrafast (<0.05 s) detection of H<sub>2</sub>O<sub>2</sub> with the limit of detection of 26 μM and sensitivity of 0.221 A M<sup>–1</sup> cm<sup>–2</sup>. Moreover, we show that ta-C/CNF hybrids can be used directly without the mass-transfer limiting polymer layers as effective immobilization platforms for glutamate oxidase for further applications in ultrafast (<0.05 s) glutamate detection. Finally, rat glial cells cultured on CNFs grown from ta-C without any additional coatings, such as polylysine, showed good adhesion on CNFs and no signs of cytotoxicity, indicating suitability of the material for future in vivo applications. This simplified and miniaturized structure provides an extremely interesting platform for various different enzyme-based electrochemical sensors.