| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Blanford, Christopher F.
University of Manchester
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (12/12 displayed)
- 2019Graphene–aramid nanocomposite fibres via superacid co-processingcitations
- 2019A Method for Metal/Protein Stoichiometry Determination Using Thin-Film Energy Dispersive X-ray Fluorescence Spectroscopycitations
- 2019Nonwoven Membrane Supports from Renewable Resources: Bamboo Fiber Reinforced Poly(Lactic Acid) Compositescitations
- 2019Nonwoven Membrane Supports from Renewable Resources: Bamboo Fiber Reinforced Poly(Lactic Acid) Compositescitations
- 2018Robust Covalently Crosslinked Polybenzimidazole/Graphene Oxide Membranes for High-Flux Organic Solvent Nanofiltrationcitations
- 2018Robust Covalently Cross-linked Polybenzimidazole/Graphene Oxide Membranes for High-Flux Organic Solvent Nanofiltrationcitations
- 2011The control of shrinkage and thermal instability in SU-8 photoresists for holographic lithographycitations
- 2008In situ high-temperature electron microscopy of 3DOM cobalt, iron oxide, and nickelcitations
- 2006Determination of void arrangements in inverse opals by transmission electron microscopycitations
- 2006The pyrolytic graphite surface as an enzyme substrate: Microscopic and spectroscopic studiescitations
- 2004A method for determining void arrangements in inverse opalscitations
- 2000Preparation and structure of 3D ordered macroporous alloys by PMMA colloidal crystal templating
Places of action
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article
A Method for Metal/Protein Stoichiometry Determination Using Thin-Film Energy Dispersive X-ray Fluorescence Spectroscopy
Abstract
A convenient approach is proposed for the quantitation of elemental cofactors in proteins and the determination of metal/protein stoichiometry, on the basis of energy dispersive X-ray fluorescence spectroscopy (EDXRF). The analysis of proteins containing the metals Cu, Fe, Zn, and Ca and also the nonmetallic element P is shown as a demonstration of the generality of the method. In general, the reported method gives limit of detection (LOD) and limit of quantification (LOQ) values in the low ppm range and requires only a few microliters of protein sample at micromolar concentrations. Moreover, sample preparation does not require any digestion steps before the analysis. The expected metal/protein stoichiometry was observed for each protein analyzed, highlighting the precision and accuracy of the method in all the tested cases. Furthermore, it is shown that the method is compatible with multimeric proteins and those with post-translational modifications such as glycosylation.