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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Merkoçi, Arben
Ministerio de Ciencia e Innovación
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (18/18 displayed)
- 2023One-Step Laser Nanostructuration of Reduced Graphene Oxide Films Embedding Metal Nanoparticles for Sensing Applicationscitations
- 2023Freestanding laser-induced two dimensional heterostructures for self-contained paper-based sensorscitations
- 2023Nanostructure Tuning of Gold Nanoparticles Films via Click Sinteringcitations
- 2023Nanostructure Tuning of Gold Nanoparticles Films via Click Sinteringcitations
- 2022Metabolomics for personalized medicine: the input of analytical chemistry from biomarker discovery to point-of-care testscitations
- 2020Chitin nanofiber paper toward optical (bio)sensing applicationscitations
- 2019Paper based photoluminescent sensing platform with recognition sites for tributyltincitations
- 2018Electrochromic molecular imprinting sensor for visual and smartphone-based detectionscitations
- 2011Variable behaviour of flexible N,O-mixed pyrazole ligand towards Zn(ii), Cd(ii) and Hg(ii) ions. Synthesis, crystal structure and fluorescent propertiescitations
- 2010Immunosensing using nanoparticlescitations
- 2010Use of Sequential Injection Analysis to construct a potentiometric electronic tongue: Application to the multidetermination of heavy metalscitations
- 2010Use of Sequential Injection Analysis to construct a potentiometric electronic tongue: Application to the multidetermination of heavy metalscitations
- 2005Integration of a glucose biosensor based on an epoxy-graphite- TTF·TCNQ-GOD biocomposite into a FIA systemcitations
- 2005Magnetically trigged direct electrochemical detection of DNA hybridization using Au67 quantum dot as electrical tracercitations
- 2005Glucose biosensor based on carbon nanotube epoxy compositescitations
- 2005Sensitive stripping voltammetry of heavy metals by using a composite sensor based on a built-in bismuth precursorcitations
- 2003Rapid electrochemical genosensor assay using a streptavidin carbon-polymer biocomposite electrodecitations
- 2003Graphite-epoxy composites as a new transducing material for electrochemical genosensingcitations
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article
Graphite-epoxy composites as a new transducing material for electrochemical genosensing
Abstract
The use of a rigid carbon-polymer composite material as an electrochemical transducer in hybridisation genosensors is reported. Graphite-epoxy composites (GEC) have an uneven surface where DNA can be adsorbed using a simple dry-adsorption procedure. Single-stranded-DNA binds strongly to GEC in a way that prevents the strands from self-associating, while permitting hybridisation with complementary DNA. Hybridisation has been detected through biotin-streptavidin interaction using a streptavidin conjugated to horseradish peroxidase. Non-specific adsorption onto GEC is almost non-existent even when the surface has not been treated by blocking reagents. The analytical signal obtained was higher when compared with other electrochemical genosensors. Results can be achieved in 150 min, and the detection limit is in the order of fmol. Additionally, surface regeneration is possible using a simple polishing procedure, allowing for multiple use. The new genosensor based on GEC fulfils the requirements desired for these devices: ease of preparation as dry-adsorption of DNA is very simple and easily automated, robustness, sensitivity, low cost of production, ease of miniaturisation and simple use and fast response. Additionally, it can be used for field measurements and can be produced as a genosensor kit. Also, this material can be implemented for screen-printing procedures for the mass production of genosensors. The utility of the genosensor based on GEC is also illustrated with the detection of a sequence related to novel determinant of β-lactamase resistance in Staphylococcus aureus. © 2003 Elsevier B.V. All rights reserved.