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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Merkoçi, Arben
Ministerio de Ciencia e Innovación
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (18/18 displayed)
- 2023One-Step Laser Nanostructuration of Reduced Graphene Oxide Films Embedding Metal Nanoparticles for Sensing Applicationscitations
- 2023Freestanding laser-induced two dimensional heterostructures for self-contained paper-based sensorscitations
- 2023Nanostructure Tuning of Gold Nanoparticles Films via Click Sinteringcitations
- 2023Nanostructure Tuning of Gold Nanoparticles Films via Click Sinteringcitations
- 2022Metabolomics for personalized medicine: the input of analytical chemistry from biomarker discovery to point-of-care testscitations
- 2020Chitin nanofiber paper toward optical (bio)sensing applicationscitations
- 2019Paper based photoluminescent sensing platform with recognition sites for tributyltincitations
- 2018Electrochromic molecular imprinting sensor for visual and smartphone-based detectionscitations
- 2011Variable behaviour of flexible N,O-mixed pyrazole ligand towards Zn(ii), Cd(ii) and Hg(ii) ions. Synthesis, crystal structure and fluorescent propertiescitations
- 2010Immunosensing using nanoparticlescitations
- 2010Use of Sequential Injection Analysis to construct a potentiometric electronic tongue: Application to the multidetermination of heavy metalscitations
- 2010Use of Sequential Injection Analysis to construct a potentiometric electronic tongue: Application to the multidetermination of heavy metalscitations
- 2005Integration of a glucose biosensor based on an epoxy-graphite- TTF·TCNQ-GOD biocomposite into a FIA systemcitations
- 2005Magnetically trigged direct electrochemical detection of DNA hybridization using Au67 quantum dot as electrical tracercitations
- 2005Glucose biosensor based on carbon nanotube epoxy compositescitations
- 2005Sensitive stripping voltammetry of heavy metals by using a composite sensor based on a built-in bismuth precursorcitations
- 2003Rapid electrochemical genosensor assay using a streptavidin carbon-polymer biocomposite electrodecitations
- 2003Graphite-epoxy composites as a new transducing material for electrochemical genosensingcitations
Places of action
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article
Rapid electrochemical genosensor assay using a streptavidin carbon-polymer biocomposite electrode
Abstract
A sensor capable of detecting a specific DNA sequence was designed by bulk modification of a graphite epoxy composite electrode with streptavidin (2% w/w). Streptavidin is used to immobilise a biotinylated capture DNA probe to the surface of the electrode. Simultaneous hybridisation occurs between the biotin DNA capture probe and the target-DNA and between the target-DNA and a digoxigenin modified probe. The rapid binding kinetic of streptavidin-biotin allows a one step immobilisation/hybridisation procedure. Secondly, enzyme labelling of the DNA duplex occurs via an antigen-antibody reaction between the Dig-dsDNA and an anti-Dig-HRP. Finally, electrochemical detection is achieved through a suitable substrate (H2O2) for the enzyme-labelled duplex. Optimisation of the sensor design, the modifier content and the immobilisation and hybridisation times was attained using a simple nucleotide sequence. Regeneration of the surface is achieved with a simple polishing procedure that shows good reproducibility. The generic use of a modified streptavidin carbon-polymer biocomposite electrode capable of surface regeneration and a one step hybridisation/immobilisation procedure are the main advantages of this approach. In DNA analysis, this procedure, if combined with the polymerase chain reaction, would represent certain advantages with respect to classical techniques, which prove to be time consuming in situations where a simple and rapid detection is required. This innovative developed material may be used for the detection of any analyte that can be coupled to the biotin-streptavidin reaction, as is the case of immunoassays. © 2003 Elsevier B.V. All rights reserved.