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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Behrends, Jan
University of Freiburg
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (19/19 displayed)
- 2023Photogeneration of spin quintet triplet–triplet excitations in DNA-assembled pentacene stackscitations
- 2023Photogeneration of Spin Quintet Triplet–Triplet Excitations in DNA-Assembled Pentacene Stacks
- 2023Photogeneration of Spin Quintet Triplet-Triplet Excitations in DNA-Assembled Pentacene Stacks.
- 2022The fast and the capacious ; a [Ni(Salen)]‐TEMPO redox‐conducting polymer for organic batteries
- 2022Spins at work: probing charging and discharging of organic radical batteries by electron paramagnetic resonance spectroscopy
- 2022Deoxyribonucleic Acid Encoded and Size-Defined π-Stacking of Perylene Diimidescitations
- 2022Deoxyribonucleic Acid Encoded and Size-Defined π-Stacking of Perylene Diimides
- 2022Deoxyribonucleic Acid Encoded and Size-Defined π-Stacking of Perylene Diimides.
- 2021Spin–spin interactions and spin delocalisation in a doped organic semiconductor probed by EPR spectroscopycitations
- 2020Expedient paramagnetic properties of surfactant-free plasmonic silicon-based nanoparticles
- 2020Correlated donor/acceptor crystal orientation controls photocurrent generation in all-polymer solar cellscitations
- 2018Reaction of porphyrin-based surface-anchored metal-organic frameworks to prolonged illumination
- 2018Reaction of porphyrin-based surface-anchored metal–organic frameworks caused by prolonged illumination
- 2016Triplet excitons as sensitive spin probes for structure analysis of extended defects in microcrystalline silicon
- 2006High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation.citations
- 2004Ion channel drug discovery and research: the automated Nano-Patch-Clamp technology.citations
- 2003Microstructured apertures in planar glass substrates for ion channel research.citations
- 2003High quality ion channel analysis on a chip with the NPC technology.citations
- 2002Whole cell patch clamp recording performed on a planar glass chip.citations
Places of action
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article
Whole cell patch clamp recording performed on a planar glass chip.
Abstract
The state of the art technology for the study of ion channels is the patch clamp technique. Ion channels mediate electrical current flow, have crucial roles in cellular physiology, and are important drug targets. The most popular (whole cell) variant of the technique detects the ensemble current over the entire cell membrane. Patch clamping is still a laborious process, requiring a skilled experimenter to micromanipulate a glass pipette under a microscope to record from one cell at a time. Here we report on a planar, microstructured quartz chip for whole cell patch clamp measurements without micromanipulation or visual control. A quartz substrate of 200 microm thickness is perforated by wet etching techniques resulting in apertures with diameters of approximately 1 microm. The apertures replace the tip of glass pipettes commonly used for patch clamp recording. Cells are positioned onto the apertures from suspension by application of suction. Whole cell recordings from different cell types (CHO, N1E-115 neuroblastoma) are performed with microstructured chips studying K(+) channels and voltage gated Ca(2+) channels.