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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Oppenheimer, Pola Goldberg
Royal Academy of Engineering
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (11/11 displayed)
- 2024Conductivity optimisation of graphene oxide-M13 bacteriophage nanocomposites: towards graphene-based gas micronano-sensorscitations
- 2024Thermonanomechanics of Graphene Oxide-M13 Bacteriophage Nanocomposites -Towards Graphene-based Nanodevicescitations
- 2022Development of Unconventional Nano‐Metamaterials from Viral Nano‐Building Blockscitations
- 2020Nanomechanics of graphene oxide-bacteriophage based self-assembled porous compositescitations
- 2016Tunable nanopatterning of conductive polymers via electrohydrodynamic lithographycitations
- 2015Influence of packing density and surface roughness of vertically-aligned carbon nanotubes on adhesive properties of gecko-inspired mimetics.
- 2014Bio-inspired hierarchical polymer fiber-carbon nanotube adhesivescitations
- 2013Hierarchical orientation of crystallinity by block-copolymer patterning and alignment in an electric fieldcitations
- 2011Carbon Nanotubes Alignment via Electrohydrodynamic Patterning of Nanocompositescitations
- 2010Rapid Electrohydrodynamic Lithography Using Low-Viscosity Polymerscitations
- 2008Preparation and Characterization of a Novel Pyrrole-benzophenone Copolymerized Silica Nanocomposite as a Reagent in a Visual Immunologic-agglutination Testcitations
Places of action
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article
Preparation and Characterization of a Novel Pyrrole-benzophenone Copolymerized Silica Nanocomposite as a Reagent in a Visual Immunologic-agglutination Test
Abstract
Biological sensing is explored through novel stable colloidal dispersions of pyrrole-benzophenone and pyrrole copolymerized silica (PPy-SiO2-PPyBPh) nanocomposites, which allow covalent linking of biological molecules through light mediation. The mechanism of nanocomposite attachment to a model protein is studied by gold labeled cholera toxin B (CTB) to enhance the contrast in electron microscopy imaging. The biological test itself is carried out without gold labeling, i.e., using CTB only. The protein is shown to be covalently bound through the benzophenone groups. When the reactive PPy-SiO2-PPyBPh-CTB nanocomposite is exposed to specific recognition anti-CTB immunoglobulins, a qualitative visual agglutination assay occurs spontaneously, producing as a positive test, PPy-SiO2-PPyBPh-CTB-anti-CTB, in less than 1 h, while the control solution of the PPy-SiO2-PPyBPh-CTB alone remained well-dispersed during the same period. These dispersions were characterized by cryogenic transmission microscopy (cryo-TEM), scanning electron microscopy (SEM), FTIR and X-ray photoelectron spectroscopy (XPS).<br/><br/>