| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Wolff, Anders
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (14/14 displayed)
- 2019Fabrication of 3D microstructure array on chip for rapid pathogen detectioncitations
- 2019Large-scale fabrication of microfluidic chips with three-dimensional microstructures for point of care application
- 2019Large-scale fabrication of microfluidic chips with three-dimensional microstructures for point of care application
- 2019A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devicescitations
- 2016Laser ablated micropillar energy directors for ultrasonic welding of microfluidic systemscitations
- 2016MICRO-SCALE ENERGY DIRECTORS FOR ULTRASONIC WELDING
- 2015Ultrasonic welding for fast bonding of self-aligned structures in lab-on-a-chip systemscitations
- 2014Fabrication and modelling of injection moulded all-polymer capillary microvalves for passive microfluidic controlcitations
- 2012A novel detection platform for parallel monitoring of DNA hybridization with high sensitivity and specificity
- 2012A novel detection platform for parallel monitoring of DNA hybridization with high sensitivity and specificity
- 2010Microfluidic DNA microarrays in PMMA chips: streamlined fabrication via simultaneous DNA immobilization and bonding activation by brief UV exposurecitations
- 2007PCR biocompatibility of Lab-on-a-chip and MEMS materialscitations
- 2006Dielectrophoresis microsystem with integrated flow cytometers for on-line monitoring of sorting efficiencycitations
- 2004Numerical simulation of travelling wave induced electrothermal fluid flowcitations
Places of action
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article
Fabrication of 3D microstructure array on chip for rapid pathogen detection
Abstract
An advanced process of free angle photolithography (FAPL) is used for making 3D supercritical angle fluorescence (SAF) structures and transfer them to the polymeric chip by injection molding for low-cost microfluidic devices with the embedded optical sensing. The FAPL was performed via a motorized stage to control the angle of incidence of light and achieve the desired shape with dimension from 50 µm to 150 µm and slope required for the 3D optical structure. These 3D structures are used for enhancement of fluorescent signal through the unique properties of SAF. The presented SAF structure has a reduced active area (50 μm) that allows enrichment of the fluorescence efficiency and reduces the amount of sample required for detection on the polymeric microfluidic chip. Herein, we are presenting reduced dimension of SAF structures, fabricated by FAPL process and increases the number of SAF per mm2 area. This also reduced the volume of sample required per test. Improvement in the limit of detections (LOD) is observed when using the small dimensions of SAF. Solid phase polymerase chain reaction (SP-PCR) on these SAF structures permits for on-chip pathogen detection. These 3D structures have the potential to be widely used in microfluidic chips as a tool for signal enrichment and low-cost point of care systems for optical detection.